Pcdp1 is a central apparatus protein that binds Ca(2+)-calmodulin and regulates ciliary motility.
Pcdp1 is a central apparatus protein that binds Ca(2+)-calmodulin and regulates ciliary motility.
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DOI：
10.1083/jcb.200912009
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发表时间：
2010-05-03
期刊：
影响因子：
--
通讯作者：
Smith EF
中科院分区：
文献类型：
--
作者：
DiPetrillo CG;Smith EF
一个复杂的，本地化到C1d中央对投射的纤毛控制鞭毛波形和节拍频率响应钙。对于所有能动的真核细胞纤毛和鞭毛，搏动是由纤毛内钙离子浓度的变化来调节的。虽然钙调节的机制尚不清楚，但大量研究表明钙调素（CaM）是轴丝钙传感器的关键。使用抗钙调素抗体和衣原体reinhardtii轴丝提取物，我们沉淀的复合物，其中包括四个多肽和特异性地与钙调素在高[Ca2+]相互作用。FAP 221是哺乳动物Pcdp1（原发性纤毛运动障碍蛋白1）的直系同源物。FAP 221和哺乳动物Pcdp1在高[Ca 2 +]时都能特异性结合CaM。降低C. reinhardtii导致C1d中心对投射组装失败和运动性的显著损害，包括不协调的弯曲、严重降低的搏动频率和改变的波形。这些综合结果表明，中央对Pcdp1（FAP 221）复合物是必不可少的纤毛运动的控制。
A complex that localizes to the C1d central pair projection of cilia controls flagellar waveform and beat frequency in response to calcium. For all motile eukaryotic cilia and flagella, beating is regulated by changes in intraciliary calcium concentration. Although the mechanism for calcium regulation is not understood, numerous studies have shown that calmodulin (CaM) is a key axonemal calcium sensor. Using anti-CaM antibodies and Chlamydomonas reinhardtii axonemal extracts, we precipitated a complex that includes four polypeptides and that specifically interacts with CaM in high [Ca2+]. One of the complex members, FAP221, is an orthologue of mammalian Pcdp1 (primary ciliary dyskinesia protein 1). Both FAP221 and mammalian Pcdp1 specifically bind CaM in high [Ca2+]. Reduced expression of Pcdp1 complex members in C. reinhardtii results in failure of the C1d central pair projection to assemble and significant impairment of motility including uncoordinated bends, severely reduced beat frequency, and altered waveforms. These combined results reveal that the central pair Pcdp1 (FAP221) complex is essential for control of ciliary motility.
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DOI：
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