Completely assembled virus particles detected by transmission electron microscopy in proximal and mid-axons of neurons infected with herpes simplex virus type 1, herpes simplex virus type 2 and pseudorabies virus.
Completely assembled virus particles detected by transmission electron microscopy in proximal and mid-axons of neurons infected with herpes simplex virus type 1, herpes simplex virus type 2 and pseudorabies virus.
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DOI：
10.1016/j.virol.2010.10.009
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发表时间：
2011-01-05
期刊：
影响因子：
3.7
通讯作者：
Friedman HM
中科院分区：
文献类型：
--
作者：
Huang J;Lazear HM;Friedman HM
在从神经元细胞体到轴突末端的顺行轴突运输过程中，α疱疹病毒的形态存在争议。报告表明，单纯疱疹病毒1型（HSV-1）核衣壳和包膜蛋白的运输发生在单独的隔室中，完整的病毒体形成在静脉曲张或轴突末端（亚组装运输模型），而相关的α疱疹病毒，伪狂犬病病毒（PRV）的运输发生在囊泡中的包膜衣壳（组装运输模型）。用透射电镜观察颈上级神经节（SCG）神经元近端和中段轴突，比较HSV-1、HSV-2和PRV的轴突顺行运输。用HSV-1 NS和17、HSV-2 2.12和PRV Becker感染SCG细胞体。完全组装的病毒颗粒内检测到细胞内的囊泡在近端和中间的轴突相邻的微管感染后，表明组装的病毒体运输顺行轴突内的所有三种α疱疹病毒。
The morphology of alphaherpesviruses during anterograde axonal transport from the neuron cell body towards the axon terminus is controversial. Reports suggest that transport of herpes simplex virus type 1 (HSV-1) nucleocapsids and envelope proteins occurs in separate compartments and that complete virions form at varicosities or axon termini (subassembly transport model), while transport of a related alphaherpesvirus, pseudorabies virus (PRV) occurs as enveloped capsids in vesicles (assembled transport model). Transmission electron microscopy of proximal and mid-axons of primary superior cervical ganglion (SCG) neurons was used to compare anterograde axonal transport of HSV-1, HSV-2 and PRV. SCG cell bodies were infected with HSV-1 NS and 17, HSV-2 2.12 and PRV Becker. Fully assembled virus particles were detected intracellularly within vesicles in proximal and mid-axons adjacent to microtubules after infection with each virus, indicating that assembled virions are transported anterograde within axons for all three alphaherpesviruses.
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影响因子：
5.4
作者：
Miranda-Saksena, Monica;Boadle, Ross A.;Cunningham, Anthony L.
通讯作者：
Cunningham, Anthony L.
影响因子：
5.4
作者：
LaVail, JH;Tauscher, AN;Sidhu, SS
通讯作者：
Sidhu, SS
影响因子：
5.4
作者：
Saksena, MM;Wakisaka, H;Cunningham, AL
通讯作者：
Cunningham, AL
影响因子：
5.4
作者：
Antinone, Sarah E.;Smith, Gregory A.
通讯作者：
Smith, Gregory A.
DOI：
10.1073/pnas.0404686101
发表时间：
2004-11-09
影响因子：
11.1
作者：
Smith, GA;Pomeranz, L;Enquist, LW
通讯作者：
Enquist, LW