Escherichia coli mar and acrAB mutants display no tolerance to simple alcohols.
Escherichia coli mar and acrAB mutants display no tolerance to simple alcohols.
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DOI：
10.3390/ijms11041403
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发表时间：
2010-03-31
影响因子：
5.6
通讯作者：
Nicholls IA
中科院分区：
文献类型：
--
作者：
Ankarloo J;Wikman S;Nicholls IA
大肠杆菌的诱导型Mar表型与对多种疏水抗生素以及一些高度疏水有机溶剂（如环己烷）的耐受性增加有关，主要通过AcrAB/TolC外排系统介导。研究了水混溶醇、乙醇和1-丙醇对大肠杆菌K-12的Mar组成突变体和Mar缺失突变体以及携带额外acrAB缺失的相应菌株的影响。与疏水溶剂相比，所有菌株在指数相被1-丙醇和乙醇杀死，其速度与亲本菌株相当。因此，Mar表型不能保护大肠杆菌免受这些极性溶剂的杀伤。令人惊讶的是，AcrAB并不会增加酒精耐受性。此外，水杨酸钠，在已知的诱导mar操纵子的浓度下，不能增加1-丙醇或乙醇耐受性。相反，两种溶剂的毒性在水杨酸钠的存在下都增加了。总的来说，结果表明，与疏水溶剂相比，大肠杆菌对水混溶醇的弹性不依赖于AcrAB/TolC外排系统，并且表明了底物分子大小和功能的下限。讨论了微生物系统在含有高含量水混溶有机溶剂的环境中的应用，例如噬菌体展示筛选。
The inducible Mar phenotype of Escherichia coli is associated with increased tolerance to multiple hydrophobic antibiotics as well as some highly hydrophobic organic solvents such as cyclohexane, mediated mainly through the AcrAB/TolC efflux system. The influence of water miscible alcohols ethanol and 1-propanol on a Mar constitutive mutant and a mar deletion mutant of E. coli K-12, as well as the corresponding strains carrying the additional acrAB deletion, was investigated. In contrast to hydrophobic solvents, all strains were killed in exponential phase by 1-propanol and ethanol at rates comparable to the parent strain. Thus, the Mar phenotype does not protect E. coli from killing by these more polar solvents. Surprisingly, AcrAB does not contribute to an increased alcohol tolerance. In addition, sodium salicylate, at concentrations known to induce the mar operon, was unable to increase 1-propanol or ethanol tolerance. Rather, the toxicity of both solvents was increased in the presence of sodium salicylate. Collectively, the results imply that the resilience of E. coli to water miscible alcohols, in contrast to more hydrophobic solvents, does not depend upon the AcrAB/TolC efflux system, and suggests a lower limit for substrate molecular size and functionality. Implications for the application of microbiological systems in environments containing high contents of water miscible organic solvents, e.g., phage display screening, are discussed.
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DOI：
10.1073/pnas.92.12.5456
发表时间：
1995-06-06
影响因子：
11.1
作者：
MARTIN, RG;ROSNER, JL
通讯作者：
ROSNER, JL
DOI：
10.1039/a708046h
发表时间：
1998-01-01
期刊：
ANALYTICAL COMMUNICATIONS
影响因子：
--
作者：
Berglund, J;Lindbladh, C;Mosbach, K
通讯作者：
Mosbach, K
影响因子：
3.2
作者：
COHEN, SP;LEVY, SB;ROSNER, JL
通讯作者：
ROSNER, JL
影响因子：
3.2
作者：
INGRAM, LO;VREELAND, NS
通讯作者：
VREELAND, NS
影响因子：
4.4
作者：
Asako, H;Nakajima, H;Aono, R
通讯作者：
Aono, R