The canine gut microbiome is associated with higher risk of gastric dilatation-volvulus and high risk genetic variants of the immune system.
The canine gut microbiome is associated with higher risk of gastric dilatation-volvulus and high risk genetic variants of the immune system.
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DOI：
10.1371/journal.pone.0197686
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Harkey MA
中科院分区：
文献类型：
--
作者：
Hullar MAJ;Lampe JW;Torok-Storb BJ;Harkey MA
大型犬和巨型犬的胃扭转（GDV）风险很高，这是一种急性，危及生命的疾病。我们小组以前的工作确定了与先天性和适应性免疫基因中的特定等位基因相关的GDV的强烈风险。我们假设免疫系统基因的变异通过调节肠道微生物组或通过自身免疫机制或两者兼而有之来使狗易患这种疾病。在这里，我们调查了犬粪便微生物组的差异是否与GDV相关，并与先前确定的风险等位基因有关。收集来自健康大丹犬（n = 38）和至少发生一次GDV的狗（n = 37）的粪便样品，并通过16S rRNA基因的配对末端测序进行分析。饮食摄入量和气质估计从研究特定的饮食和气质问卷。患有GDV的狗的粪便微生物组比健康对照狗显著更多样化。在患有GDV的狗中，以及具有至少一个DRB 1和TRL5风险等位基因的狗中，α多样性显著增加。我们没有发现饮食摄入量和GDV的显着关联。患有GDV的狗显示稀有放线菌谱系的显著扩增（p = 0.004），以及厚壁菌门的显著更高丰度（p = 0.004）和拟杆菌门的显著更低丰度（p<0.004）。有10个属的丰度有显着差异，但经多重比较校正后，没有显着。细菌门在对照组和患有GDV和至少一个DRB 1和TRL5风险等位基因的狗之间存在显著差异。放线菌显着较高的狗与GDV和一个危险等位基因DRB 1和TLR5，但不是DLA 88基因。此外，在DRB 1和TLR 5至少有一个危险等位基因的犬中，柯林斯氏菌显著增加。Logistic回归显示，包括放线菌、至少一个风险等位基因和气质的模型解释了大丹犬GDV风险变化的29%。GDV中的微生物组通过次要谱系的扩展而改变，并且与先天性和适应性免疫基因的特定等位基因相关。这些关联与我们的假设一致，即免疫基因可能通过改变肠道微生物组在GDV易感性中发挥作用。需要进一步的研究来直接测试免疫基因、肠道微生物组和GDV之间的因果关系。
Large and giant dog breeds have a high risk for gastric dilatation-volvulus (GDV) which is an acute, life-threatening condition. Previous work by our group identified a strong risk of GDV linked to specific alleles in innate and adaptive immune genes. We hypothesize that variation in the genes of the immune system act through modulation of the gut microbiome, or through autoimmune mechanisms, or both, to predispose dogs to this condition. Here, we investigate whether differences in the canine fecal microbiome are associated with GDV and are linked to previously identified risk alleles. Fecal samples from healthy Great Danes (n = 38), and dogs with at least one occurrence of GDV (n = 37) were collected and analyzed by paired-end sequencing of the 16S rRNA gene. Dietary intake and temperament were estimated from a study-specific dietary and temperament questionnaire. Dogs with GDV had significantly more diverse fecal microbiomes than healthy control dogs. Alpha diversity was significantly increased in dogs with GDV, as well as dogs with at least one risk allele for DRB1 and TRL5. We found no significant association of dietary intake and GDV. Dogs with GDV showed a significant expansion of the rare lineage Actinobacteria (p = 0.004), as well as a significantly greater abundance of Firmicutes (p = 0.004) and a significantly lower abundance of Bacteroidetes (p<0.004). There was a significant difference in the abundance of 10 genera but after correction for multiple comparisons, none were significant. Bacterial phyla were significantly different between controls and dogs with GDV and at least one risk allele for DRB1 and TRL5. Actinobacteria were significantly higher in dogs with GDV and with one risk allele for DRB1 and TLR5 but not DLA88 genes. Furthermore, Collinsella was significantly increased in dogs with at least one risk allele for DRB1 and TLR5. Logistic regression showed that a model which included Actinobacteria, at least one risk allele,and temperament, explained 29% of the variation in risk of GDV in Great Danes. The microbiome in GDV was altered by an expansion of a minor lineage and was associated with specific alleles of both innate and adaptive immunity genes. These associations are consistent with our hypothesis that immune genes may play a role in predisposition to GDV by altering the gut microbiome. Further research will be required to directly test the causal relationships of immune genes, the gut microbiome and GDV.
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影响因子：
5.6
作者：
Fu BC;Randolph TW;Lim U;Monroe KR;Cheng I;Wilkens LR;Le Marchand L;Hullar MA;Lampe JW
通讯作者：
Lampe JW
影响因子：
3.7
作者：
Gomez A;Luckey D;Yeoman CJ;Marietta EV;Berg Miller ME;Murray JA;White BA;Taneja V
通讯作者：
Taneja V
影响因子：
2.2
作者：
Di Bella, Julia M.;Bao, Yige;Reid, Gregor
通讯作者：
Reid, Gregor
DOI：
10.5326/15473317-33-3-197
发表时间：
1997-05-01
影响因子：
1.3
作者：
Glickman, LT;Glickman, NW;Lantz, GC
通讯作者：
Lantz, GC
DOI：
10.1016/j.clim.2015.04.016
发表时间：
2015-08
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
--
作者：
Gomez A;Luckey D;Taneja V
通讯作者：
Taneja V