Values of a novel pyroptosis-related genetic signature in predicting outcome and immune status of pancreatic ductal adenocarcinoma.
Values of a novel pyroptosis-related genetic signature in predicting outcome and immune status of pancreatic ductal adenocarcinoma.
复制标题
DOI：
10.1093/gastro/goac051
复制
发表时间：
2022
影响因子：
3.6
通讯作者：
Yin, Xiao-Yu
中科院分区：
文献类型：
--
作者：
Xu, Xiang;Liang, Jia-Hua;Li, Jian-Hui;Xu, Qiong-Cong;Yin, Xiao-Yu
上睑下垂是一种新出现的与恶性肿瘤进展相关的程序性细胞死亡。然而，关于胰腺导管腺癌(PDAC)和上睑下垂之间的关系的研究报道很少。因此，我们的目标是构建一个与上睑下垂相关的基因特征，以预测PDAC患者的临床结果和免疫状态。对来自癌症基因组图谱(TCGA)的176例PDAC患者和来自基因-组织表达计划的167例PDAC患者的RNA-SEQ数据进行了分析，以寻找PDAC和正常胰腺之间与上睑下垂相关的差异表达基因(DEG)。使用最小绝对收缩和选择算子(LASSO)COX回归分析DEGS的风险特征，其准确性在基因表达总括(GEO)队列(n = 190)中得到验证。通过功能富集化分析探讨了DEGS的作用机制。用单样本基因集浓缩分析和估计算法对每组的免疫特性进行评估。Lasso Cox回归分析产生了九个基因的风险特征，并根据中位风险分数将PDAC患者分为高风险组或低风险组。高风险组的总体生存时间明显短于低风险组，这一点在外部GEO数据库中得到了验证。构建了基于风险特征的诺模图，具有较好的预测性能。功能浓缩分析表明，上睑下垂可能调节了PDAC的肿瘤免疫微环境。免疫渗入评估显示，低危组的免疫状态比高危组更活跃。包含9个上睑下垂相关基因的风险标志可能是PDAC的预后标志。上睑下垂可能通过调节肿瘤免疫微环境影响PDAC患者的预后。
Pyroptosis is an emerging form of programmed cell death associated with progression in malignancies. Yet, there are few studies reporting on the association between pancreatic ductal adenocarcinoma (PDAC) and pyroptosis. Therefore, we aimed to construct a pyroptosis-related genetic signature to predict the clinical outcome and immune status in PDAC patients. RNA-seq data of 176 PDAC patients from The Cancer Genome Atlas (TCGA) and 167 PDAC patients from the Genotype-Tissue Expression Project were analysed for pyroptosis-related differentially expressed genes (DEGs) between PDAC and normal pancreas. The risk signature of DEGs was analysed using the least absolute shrinkage and selection operator (LASSO) Cox regression analysis and its accuracy was validated in the Gene Expression Omnibus (GEO) cohort (n = 190). Functional enrichment analyses were performed to explore the mechanisms of the DEGs. The immune characteristics were evaluated using single-sample gene set enrichment analysis and ESTIMATE algorithms for each group. A nine-gene risk signature was generated from LASSO Cox regression analysis and classified PDAC patients into either a high- or low-risk group according to the median risk score. The high-risk group had significantly shorter overall survival than the low-risk group and it was verified in the external GEO database. A nomogram based on the risk signature was constructed and showed an ideal prediction performance. Functional enrichment analyses revealed that pyroptosis might regulate the tumor immune microenvironment in PDAC. Immune infiltration evaluation suggested that immune status was more activated in the low-risk group than in the high-risk group. The risk signature encompassing nine pyroptosis-related genes may be a prognostic marker for PDAC. Pyroptosis might affect the prognosis of PDAC patients via regulating the tumor immune microenvironment.
登录
查看更多内容
影响因子：
3.7
作者：
Shi H;Zhong F;Yi X;Shi Z;Ou F;Xu Z;Zuo Y
通讯作者：
Zuo Y
影响因子：
5.8
作者：
Tang R;Liu X;Wang W;Hua J;Xu J;Liang C;Meng Q;Liu J;Zhang B;Yu X;Shi S
通讯作者：
Shi S
影响因子：
4
作者：
Modi S;Kir D;Banerjee S;Saluja A
通讯作者：
Saluja A
肿瘤纯度作为宫颈癌的预后和免疫治疗相关特征
DOI：
10.18632/aging.203714
发表时间：
2021-11-29
期刊：
Aging
影响因子：
--
作者：
Deng Y;Song Z;Huang L;Guo Z;Tong B;Sun M;Zhao J;Zhang H;Zhang Z;Li G
通讯作者：
Li G
影响因子：
50.3
作者：
Bear AS;Vonderheide RH;O'Hara MH
通讯作者：
O'Hara MH