Hypertonic saline inhibits arachidonic acid priming of the human neutrophil oxidase.
Hypertonic saline inhibits arachidonic acid priming of the human neutrophil oxidase.
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DOI：
10.1016/j.jss.2011.06.022
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发表时间：
2012-05-01
期刊：
影响因子：
--
通讯作者：
Silliman CC
中科院分区：
文献类型：
--
作者：
Lee L;Kelher MR;Moore EE;Banerjee A;Silliman CC
花生四烯酸（AA）及其白三烯衍生物，例如：LTB 4）是休克后肠系膜淋巴中的炎性介质，其似乎作为G蛋白偶联受体（GPCR）的激动剂起作用。这些介质使中性粒细胞（PMNs）增加超氧化物的产生，这与ALI的发生有关。高渗盐水（HTS）也已被证明具有免疫调节作用，如通过排除适当的网格蛋白介导的激活GPCR的内吞作用来减弱PMN引发，从而潜在地减弱ALI。我们推测高温超导抑制这些脂质介质引发的中性粒细胞氧化酶。从健康供体分离出PMN后，在37°C下在等渗缓冲液（对照）或HTS（180 mmol/L）中孵育5分钟。然后用AA [5 μM]预处理PMN 10分钟或用LTB 4 [1 μM]预处理5分钟，并用200 ng/ml佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA，一种非GPCR激活剂）激活氧化酶，并通过还原细胞色素c测量超氧阴离子的产生。AA [5 μM]和LTB 4 [1 μM]均显著引发PMA激活的呼吸爆发（p<0.05，ANOVA，Newman-Keuls，n=4）。HTS抑制AA和LTB 4引发的呼吸爆发。这些数据表明，HTS降低这些脂质介质在体外刺激的中性粒细胞的细胞毒性，并进一步支持HTS的免疫调节作用。
Arachidonic acid (AA, and its leukotriene derivatives e.g.: LTB4) is an inflammatory mediator in post-shock mesenteric lymph that appears to act as an agonist on G-protein coupled receptors (GPCRs). These mediators prime neutrophils (PMNs) for an increased production of superoxide, implicated in the development of ALI. Hypertonic saline (HTS) has also been shown to have immunomodulatory effects such as attenuation of PMN priming by precluding appropriate clathrin-mediated endocytosis of activated GPCRs, thereby potentially attenuating ALI. We hypothesize that HTS inhibits priming of the PMN oxidase by these lipid mediators. After PMNs were isolated from healthy donors, incubation was done in either isotonic buffer (control) or HTS (180 mmol/L) for 5 minutes at 37°C. The PMNs were then primed for 10 minutes with AA [5 μM] or 5 minutes with LTB4 [1 μM] and the oxidase was activated with 200 ng/ml of phorbol 12-myristate 13-acetate (PMA), a non-GPCR activator, and superoxide anion generation was measured via reduction of cytochrome c. Both AA [5 μM] and LTB4 [1 μM] significantly primed the PMA activated respiratory burst (p<0.05, ANOVA, Newman-Keuls, n=4). HTS inhibited both AA and LTB4 priming of the respiratory burst. These data indicate that HTS reduces the cytotoxicity of PMNs stimulated by these lipid mediators in vitro and further support the immunomodulatory effects of HTS.
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DOI：
10.1152/ajpgi.1995.268.3.g397
发表时间：
1995-03-01
影响因子：
4.5
作者：
KOIKE, K;MOORE, EE;BANERJEE, A
通讯作者：
BANERJEE, A
DOI：
10.1042/bj20091087
发表时间：
2010-11-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Khan SY;McLaughlin NJ;Kelher MR;Eckels P;Gamboni-Robertson F;Banerjee A;Silliman CC
通讯作者：
Silliman CC
DOI：
10.1016/j.bbrc.2010.02.173
发表时间：
2010-05-21
影响因子：
3.1
作者：
Radmark, Olof;Samuelsson, Bengt
通讯作者：
Samuelsson, Bengt
影响因子：
3.8
作者：
Ciesla, DJ;Moore, EE;Sauaia, A
通讯作者：
Sauaia, A
影响因子：
5.5
作者：
Grenier, S;Flamand, N;Bourgoin, SG
通讯作者：
Bourgoin, SG