Mesangial cell expression of proto-oncogene Ets-1 during progression of mesangioproliferative glomerulonephritis.
Mesangial cell expression of proto-oncogene Ets-1 during progression of mesangioproliferative glomerulonephritis.
复制标题
系膜增生性肾小球肾炎进展过程中原癌基因 Ets-1 的系膜细胞表达。
DOI：
10.1111/j.1523-1755.2004.00782.x
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发表时间：
2004
影响因子：
19.6
通讯作者：
P. Mertens
中科院分区：
文献类型：
--
作者：
U. Raffetseder;N. Wernert;T. Ostendorf;Claudia R C van Roeyen;T. Rauen;P. Behrens;J. Floege;P. Mertens
BACKGROUND
Ets-1 is a transactivator of matrix-associated genes and key factor in neoangiogenesis. The expression of Ets-1 mRNA and protein was analyzed in healthy rat kidney and in a model for mesangioproliferative disease without and with inhibition of platelet-derived growth factor-B (PDGF-B) activity.
METHODS
Immunohistochemistry was performed using a specific noncrossreacting anti-Ets-1 antibody and included a counterstain with alpha-smooth muscle actin (alpha-SMA). Nuclear proteins from isolated glomeruli were analyzed by Western blotting. Changes in Ets-1 mRNA levels were detected by in situ hybridization and Northern blotting. PDGF-B antagonism was performed in nephritic rats by specific aptamers.
RESULTS
The distribution of Ets-1-positive cells in healthy rats was heterogenous with exclusively nuclear staining of glomerular, tubular, and vascular cells. Profound changes were detected in the anti-Thy 1.1 glomerulonephritis. Nuclear Ets-1 staining was intense in mesangial cells, whereas podocyte and endothelial cell staining was unchanged. The strongest signal was seen on days 2 to 7. By Western blotting of glomerular proteins a single 52 kD band was detected in healthy rats, which was increased 4.5-fold after disease induction. At the same time a 54 kD band appeared that most likely represents phosphorylated Ets-1. Ets-1 transcripts were detected in mesangial cells that include exon IV but lack exon VII. A concordant 6.4-fold up-regulation of mRNA was detected in glomeruli. Specific PDGF-B antagonism by aptamer treatment from days 3 to 7 after disease induction led to reduced Ets-1 expression on day 7, correlating with decreased mesangial cell numbers.
CONCLUSION
A temporal increase of mesangial cell Ets-1 expression correlates with mesangial cell activation in mesangioproliferative glomerulonephritis. PDGF-B may partially contribute to the increased expression.
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影响因子：
19.6
作者：
Kliem, V;Johnson, RJ;Floege, J
通讯作者：
Floege, J
明胶酶 A 是一种肾小球系膜细胞生长和分化因子。
影响因子：
19.6
作者：
Turck,J;Pollock,AS;Lovett,DH
通讯作者：
Lovett,DH
DOI：
10.1152/ajpcell.1998.274.2.c472
发表时间：
1998-02-01
影响因子：
5.5
作者：
Naito, S;Shimizu, S;Fagin, JA
通讯作者：
Fagin, JA
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DOI：
--
发表时间：
1990
期刊：
The American journal of pathology
影响因子：
--
作者：
Johnson,RJ;Garcia,RL;Pritzl,P;Alpers,CE
通讯作者：
Alpers,CE
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DOI：
--
发表时间：
1992
期刊：
The American journal of pathology
影响因子：
--
作者：
Lovett,DH;Johnson,RJ;Marti,HP;Martin,J;Davies,M;Couser,WG
通讯作者：
Couser,WG