Localization of plasma membrane bound NTPDases in the murine reproductive tract.
Localization of plasma membrane bound NTPDases in the murine reproductive tract.
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鼠类生殖道中质膜结合的NTPDase的定位。
DOI：
10.1007/s00418-008-0551-3
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发表时间：
2009-05
影响因子：
2.3
通讯作者：
Sévigny J
中科院分区：
文献类型：
--
作者：
Martín-Satué M;Lavoie EG;Pelletier J;Fausther M;Csizmadia E;Guckelberger O;Robson SC;Sévigny J
细胞外核苷酸可能影响生殖生物学的各个方面，因为ATP影响平滑肌收缩，参与类固醇生成和精子发生，并且还调节输卵管中的跨上皮转运。细胞核苷酸嘌呤能受体的激活受胞外核苷三磷酸二磷酸水解酶（E-NTPDase）家族的4个质膜结合成员的影响，即NTPDase 1、NTPDase 2、NTPDase 3和NTPDase 8，它们的胞外酶性质不同。本研究的目的是通过免疫学技术（免疫标记，蛋白质印迹）和酶促测定，原位和组织匀浆的膜结合NTPDases在小鼠雌性和雄性生殖道的表达谱的特征。除了在血管内皮细胞和平滑肌细胞上的预期表达外，还在睾丸的支持细胞和间质巨噬细胞、卵巢颗粒细胞和附睾上皮的顶端细胞中检测到NTPDase 1。NTPDase 2主要由结缔组织中的细胞表达; NTPDase 3在分泌上皮中表达，最后，NTPDase 8在本文研究的任何组织中均未检测到。此外，NTPD酶6在圆形精子细胞的高尔基期顶体囊泡中被检测到。这项描述性研究表明，NTPD酶密切调节生殖道细胞外核苷酸水平，可能会影响特定的生物学功能。
Extracellular nucleotides might influence aspects of the biology of reproduction in that ATP affects smooth muscle contraction, participates in steroidogenesis and spermatogenesis, and also regulates transepithelial transport, as in oviducts. Activation of cellular nucleotide purinergic receptors is influenced by four plasma membrane-bound members of the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family, namely NTPDase1, NTPDase2, NTPDase3, and NTPDase8 that differ in their ecto-enzymatic properties. The purpose of this study was to characterize the expression profile of the membrane-bound NTPDases in the murine female and male reproductive tracts by immunological techniques (immunolabelling, Western blotting) and by enzymatic assays, in situ and on tissue homogenates. Other than the expected expression on vascular endothelial and smooth muscle cells, NTPDase1 was also detected in Sertoli cells and interstitial macrophages in testes, in ovarian granulosa cells, and in apical cells from epididymal epithelium. NTPDase2 was largely expressed by cells in the connective tissue; NTPDase3 in secretory epithelia, and finally, NTPDase8 was not detected in any of the tissues studied here. In addition, NTPDase6 was putatively detected in Golgi-phase acrosome vesicles of round spermatids. This descriptive study suggests close regulation of extracellular nucleotide levels in the genital tract by NTPDases that may impact specific biological functions.
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影响因子：
4.1
作者：
Braun, N;Fengler, S;Zimmermann, H
通讯作者：
Zimmermann, H
影响因子：
2.9
作者：
Esch, JSA;Sévigny, J;Robson, SC
通讯作者：
Robson, SC
影响因子：
6.7
作者：
Banks, FCL;Knight, GE;Burnstock, G
通讯作者：
Burnstock, G
DOI：
10.1590/s0100-879x2001001000003
发表时间：
2001-10-01
影响因子：
2.3
作者：
Casali, EA;da Silva, TR;Bernard, EA
通讯作者：
Bernard, EA
影响因子：
4.8
作者：
FILIPPINI, A;RICCIOLI, A;ZIPARO, E
通讯作者：
ZIPARO, E