Membrane curvature and connective fiber alignment in guinea pig round window membrane.
Membrane curvature and connective fiber alignment in guinea pig round window membrane.
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DOI：
10.1016/j.actbio.2021.09.036
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发表时间：
2021-12
影响因子：
9.7
通讯作者：
Kysar JW
中科院分区：
文献类型：
--
作者：
Arriaga M;Arteaga DN;Fafalis D;Yu M;Wang X;Kasza KE;Lalwani AK;Kysar JW
圆窗膜（RWM）覆盖在充满外淋巴液的内耳空间和充满空气的中耳空间之间的开口。作为这两个空间之间唯一的非骨屏障，RWM是用于诊断目的的外淋巴液抽吸和用于治疗内耳疾病的药物输送的理想候选者。常规进入RWM需要开发新的手术工具，其设计只有在彻底了解RWM的结构和特性的情况下才能进行优化。RWM具有胶原蛋白和弹性纤维层，因此表征这些纤维的分布和取向是必不可少的。在豚鼠模型中对完整RWM进行共聚焦和双光子显微镜检查，以表征胶原和弹性纤维的分布。通过二次谐波产生、自体荧光和罗丹明B染色对纤维成像。RWM的纤维取向和几何特性的定量分析揭示了在RWM的某些部分中平均纤维取向和零曲率方向之间的显著相关性，在近似平行于耳蜗轴的方向上，平均纤维取向和沿沿着RWM的线性距离之间的相关性甚至更显著。所测量的平均纤维方向和分散可以被合并到广义结构张量中，用于开发连续各向异性机械本构模型，这反过来将使得能够优化手术工具以进入耳蜗。
The round window membrane (RWM) covers an opening between the perilymph fluid-filled inner ear space and the air-filled middle ear space. As the only non-osseous barrier between these two spaces, the RWM is an ideal candidate for aspiration of perilymph for diagnostics purposes and delivery of medication for treatment of inner ear disorders. Routine access across the RWM requires the development of new surgical tools whose design can only be optimized with a thorough understanding of the RWM’s structure and properties. The RWM possesses a layer of collagen and elastic fibers so characterization of the distribution and orientation of these fibers is essential. Confocal and two-photon microscopy were conducted on intact RWMs in a guinea pig model to characterize the distribution of collagen and elastic fibers. The fibers were imaged via second-harmonic-generation, autofluorescence, and Rhodamine B staining. Quantitative analyses of both fiber orientation and geometrical properties of the RWM uncovered a significant correlation between mean fiber orientations and directions of zero curvature in some portions of the RWM, with an even more significant correlation between the mean fiber orientations and linear distance along the RWM in a direction approximately parallel to the cochlear axis. The measured mean fiber directions and dispersions can be incorporated into a generalized structure tensor for use in the development of continuum anisotropic mechanical constitutive models that in turn will enable optimization of surgical tools to access the cochlea.
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