Multiplexed salivary protein profiling for patients with respiratory diseases using fiber-optic bundles and fluorescent antibody-based microarrays.
Multiplexed salivary protein profiling for patients with respiratory diseases using fiber-optic bundles and fluorescent antibody-based microarrays.
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DOI：
10.1021/ac4019523
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发表时间：
2013-10-01
影响因子：
7.4
通讯作者：
Walt, David R.
中科院分区：
文献类型：
--
作者：
Nie, Shuai;Benito-Pena, Elena;Zhang, Huaibin;Wu, Yue;Walt, David R.
在过去40年中，呼吸道疾病的发病率和流行率在世界各地显著增加，损害了经济生产力，并对卫生保健系统提出了挑战。目前对不同呼吸道疾病的诊断通常涉及侵入性采样方法，例如诱导痰或支气管肺泡灌洗，这对患者来说是不舒服的，甚至是痛苦的。在本文中，我们提出了一个平台，将光纤束和抗体为基础的微阵列进行多重蛋白质分析的一组六个唾液生物标志物的哮喘和囊性纤维化（CF）的诊断。该平台利用包含约50，000个单独的4.5 μm直径纤维的光纤束，这些纤维被化学蚀刻以产生微孔，其中可以沉积用单克隆捕获抗体修饰的修饰微球。基于夹心免疫测定形式，该阵列定量亚皮摩尔范围内的人血管内皮生长因子（VEGF）、干扰素γ诱导蛋白10（IP-10）、白细胞介素8（IL-8）、表皮生长因子（EGF）、基质金属蛋白酶9（MMP-9）和白细胞介素1 β（IL-1β）唾液生物标志物。在平台上分析了从291个个体（164名哮喘患者、71名CF患者和56名健康对照（HC））收集的唾液上清液，以使用该六分析物套件对每组患者进行分析。结果发现，与HC相比，在哮喘和CF患者中观察到六种蛋白质中的四种显著升高（p<0.01）。这些结果证明了使用多重蛋白质阵列平台进行呼吸系统疾病诊断的潜力。
Over the past 40 years, the incidence and prevalence of respiratory diseases have increased significantly throughout the world, damaging economic productivity and challenging health care systems. Current diagnoses of different respiratory diseases generally involve invasive sampling methods such as induced sputum or bronchoalveolar lavage that are uncomfortable, or even painful, for the patient. In this paper, we present a platform incorporating fiber-optic bundles and antibody based microarrays to perform multiplexed protein profiling of a panel of six salivary biomarkers for asthma and cystic fibrosis (CF) diagnosis. The platform utilizes an optical fiber bundle containing approximately 50,000 individual 4.5 μm diameter fibers that are chemically etched to create microwells in which modified microspheres decorated with monoclonal capture antibodies can be deposited. Based on a sandwich immunoassay format, the array quantifies human vascular endothelial growth factor (VEGF), interferon gamma-induced protein 10 (IP-10), interleukin 8 (IL-8), epidermal growth factor (EGF), matrix metalloproteinase 9 (MMP-9), and interleukin 1 beta (IL-1β) salivary biomarkers in the sub-picomolar range. Saliva supernatants collected from 291 individuals (164 asthmatics, 71 CF patients, and 56 healthy controls (HC)) were analyzed on the platform to profile each group of patients using this six-analyte suite. It was found that four of the six proteins were observed to be significantly elevated (p<0.01) in asthma and CF patients compared with HC. These results demonstrate the potential to use the multiplexed protein array platform for respiratory disease diagnosis.
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Walt, David R.
DOI：
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发表时间：
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影响因子：
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