Microarray based comparison of two Escherichia coli O157:H7 lineages.
Microarray based comparison of two Escherichia coli O157:H7 lineages.
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DOI：
10.1186/1471-2180-6-30
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发表时间：
2006-03-15
期刊：
影响因子：
4.2
通讯作者：
Ishizaki H
中科院分区：
文献类型：
--
作者：
Dowd SE;Ishizaki H
先前的研究已经确定了大肠杆菌O157：H7存在两个独立谱系的可能性。临床分离株往往主要聚集在这两个谱系之一中。为了确定是否有毒力相关基因差异表达的两个谱系之间，我们选择利用微阵列技术进行初步筛选。利用610基因芯片，设计了针对E。coli O157 EDL 933转录组，主要针对毒力系统，我们选择了3个代表性的谱系I分离株（LI组主要是临床分离株）和3个代表性的谱系II分离株（LII组主要是牛分离株）。使用标准染料交换实验设计，揭示了两个谱系之间73个基因的统计学差异表达（P < 0.05）。结果强调表明，在体外厌氧生长条件下，在谱系I中存在stx2b、ureD、curli（csgAFEG）和应激相关基因（hslJ、cspG、ibpB、ibpA）的上调，这可能有助于增强毒力或传播潜力。谱系II表现出III型分泌器、LPS和鞭毛相关转录物的显著上调。这些结果提供了深入了解毒力基因的比较调控，以及为未来的研究提供了方向。最后，对不同菌株中关键毒力因子的表达进行了评价。大肠杆菌O157分离株具有内在价值，随着我们对毒力、致病性和传播的理解的提高，对这些表达数据的解释将继续发展。
Previous research has identified the potential for the existence of two separate lineages of Escherichia coli O157:H7. Clinical isolates tended to cluster primarily within one of these two lineages. To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening. Using a 610 gene microarray, designed against the E. coli O157 EDL 933 transcriptome, targeting primarily virulence systems, we chose 3 representative Lineage I isolates (LI groups mostly clinical isolates) and 3 representative Lineage II isolates (LII groups mostly bovine isolates). Using standard dye swap experimental designs, statistically different expression (P < 0.05) of 73 genes between the two lineages was revealed. Result highlights indicate that under in vitro anaerobic growth conditions, there is up-regulation of stx2b, ureD, curli (csgAFEG), and stress related genes (hslJ, cspG, ibpB, ibpA) in Lineage I, which may contribute to enhanced virulence or transmission potential. Lineage II exhibits significant up-regulation of type III secretion apparatus, LPS, and flagella related transcripts. These results give insight into comparative regulation of virulence genes as well as providing directions for future research. Ultimately, evaluating the expression of key virulence factors among different E. coli O157 isolates has inherent value and the interpretation of such expression data will continue to evolve as our understanding of virulence, pathogenesis and transmission improves.
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DOI：
10.1016/s0006-291x(03)00901-x
发表时间：
2003-06-13
影响因子：
3.1
作者：
Kimura, T;Tani, S;Matsumoto, Y
通讯作者：
Matsumoto, Y
影响因子：
3.1
作者：
Blomgran, R;Zheng, LM;Stendahl, O
通讯作者：
Stendahl, O
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
64.5
作者：
Artsimovitch, I;Landick, R
通讯作者：
Landick, R
影响因子：
4.2
作者：
Besser, TE;Richards, BL;Hancock, DD
通讯作者：
Hancock, DD