Enriched environment and stress exposure influence splenic B lymphocyte composition.
Enriched environment and stress exposure influence splenic B lymphocyte composition.
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丰富的环境和压力暴露影响脾 B 淋巴细胞的组成。
DOI：
10.1371/journal.pone.0180771
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Bhargava A
中科院分区：
文献类型：
--
作者：
Gurfein BT;Hasdemir B;Milush JM;Touma C;Palme R;Nixon DF;Darcel N;Hecht FM;Bhargava A
长期的慢性压力对免疫功能有有害影响，并与许多负面的健康结果有关。脾脏容纳了身体四分之一的淋巴细胞，并介导先天性和适应性免疫反应。然而，脾淋巴细胞的子集，无论是适应性或适应不良，各种压力仍然在很大程度上是未知的。在这里，我们研究了不可预测的慢性轻度应激（CMS）暴露对雄性小鼠脾脏组成的影响，这些小鼠被关在两种不同的笼子里：标准笼子（Cntl）和丰富的环境（EE）。EE笼养小鼠表现出最大的脾细胞绝对数量，并且CMS暴露显着降低了两种笼养条件下的脾细胞数量。通过平均粪便皮质酮代谢物（FCM）测量的糖皮质激素产生在EE笼小鼠中显著低于Cntl笼小鼠。令人惊讶的是，CMS暴露导致EE-笼小鼠中平均FCM的增加，但在Cntl-笼小鼠中没有显著变化。CMS改变了脾B：T淋巴细胞的比例;它降低了B细胞的频率，但增加了EE笼小鼠T细胞的频率。脾细胞数和B：T淋巴细胞比值与平均FCM呈负相关。EE笼小鼠的未成熟和成熟B细胞的频率低于Cntl笼小鼠。CMS显著增加未成熟和边缘区B细胞的频率，但在两种笼条件下降低滤泡B细胞的频率。平均FCM与未成熟、边缘区和生发中心B细胞的频率呈正相关，而与滤泡B细胞的频率呈负相关。总之，脾免疫细胞，特别是B淋巴细胞的组成，调制笼环境和压力，并可能引发小鼠不同的免疫挑战。
Prolonged chronic stress has deleterious effects on immune function and is associated with numerous negative health outcomes. The spleen harbors one-fourth of the body’s lymphocytes and mediates both innate and adaptive immune responses. However, the subset of splenic lymphocytes that respond, either adaptively or maladaptively, to various stressors remains largely unknown. Here we investigated the effects of unpredictable chronic mild stress (CMS) exposure on spleen composition in male mice housed in two different caging conditions: standard caging (Cntl) and enriched environment (EE). EE-caged mice exhibited the greatest absolute number of splenocytes and CMS exposure significantly lowered splenocyte numbers in both caging conditions. Glucocorticoid production, measured by mean fecal corticosterone metabolites (FCM), was significantly lower in EE-caged mice vs. Cntl-caged mice. Surprisingly, CMS exposure resulted in an increase in mean FCM in EE-caged mice, but no significant change in Cntl-caged mice. CMS altered the splenic B:T lymphocyte ratio; it reduced the frequency of B cells, but increased the frequency of T cells in EE-caged mice. Splenocyte number and B:T lymphocyte ratio showed a negative relationship with mean FCM. EE-caged mice had a lower frequency of immature and germinal B cells than Cntl-caged mice. CMS markedly increased the frequency of immature and marginal zone B cells, but decreased the frequency of follicular B cells in both caging conditions. Mean FCM correlated positively with frequency of immature, marginal zone and germinal center B cells, but negatively with frequency of follicular B cells. To conclude, splenic immune cells, particularly B lymphocyte composition, are modulated by caging environment and stress and may prime mice differently to respond to immune challenges.
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DOI：
10.1084/jem.191.8.1423
发表时间：
2000-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Yin D;Tuthill D;Mufson RA;Shi Y
通讯作者：
Shi Y
DOI：
10.1016/j.bbi.2014.05.016
发表时间：
2014-10
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Miller GE;Murphy ML;Cashman R;Ma R;Ma J;Arevalo JM;Kobor MS;Cole SW
通讯作者：
Cole SW
影响因子：
10.6
作者：
YEHUDA, R;BOISONEAU, D;GILLER, EL
通讯作者：
GILLER, EL
影响因子：
2.9
作者：
SANCHEZ, ER;FABER, LE;PRATT, WB
通讯作者：
PRATT, WB
影响因子：
15.1
作者：
Johnson, JD;O'Connor, KA;Maier, SF
通讯作者：
Maier, SF