Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model.
Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model.
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DOI：
10.1111/wrr.12600
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Pastar I
中科院分区：
文献类型：
--
作者：
Glinos GD;Verne SH;Aldahan AS;Liang L;Nouri K;Elliot S;Glassberg M;Cabrera DeBuc D;Koru-Sengul T;Tomic-Canic M;Pastar I
离体人体皮肤伤口模型是研究伤口上皮化的广泛接受的模型。由于缺乏完全复制人类状况的动物模型，离体模型是研究伤口上皮再生机制以及新疗法的临床前测试的宝贵工具。目前评估该模型伤口愈合的标准是组织形态学分析，这是劳动密集型、耗时的，并且除了不同时间点的评估之外还需要多次生物学和技术重复。光学相干断层扫描 (OCT) 是一种新兴的非侵入性成像技术，最初是为非侵入性视网膜扫描而开发的，可避免组织处理的有害影响。这项研究调查了 OCT 作为一种评估人类离体伤口模型中上皮再形成的新方法。产生离体切除伤口，将其维持在空气-液体界面，并在第4天和第7天用OCT和组织学评估愈合进展。 OCT 提供了足够的分辨率来识别表皮、乳头状和网状真皮，以及重要的是伤口床上的迁移上皮。我们将 OCT 作为一种非侵入性工具，用于对离体人体伤口愈合过程进行纵向“光学活检”，并基于整个伤口区域的正面 OCT 图像建立了上皮再形成的最佳量化方法。 OCT 的成对统计分析和基于组织学的上皮化率定量显示了 OCT 技术用于无创监测人体伤口上皮化的可行性和优越性。此外，我们利用 OCT 来评估同种异体脂肪干细胞的治疗潜力，揭示它们促进人类离体伤口上皮再形成的能力。 OCT 技术在实验室和临床环境中伤口愈合和新型疗法评估方面的应用前景广阔。
The ex vivo human skin wound model is a widely accepted model to study wound epithelialization. Due to a lack of animal models that fully replicate human conditions, the ex vivo model is a valuable tool to study mechanisms of wound re-epithelialization, as well as for pre-clinical testing of novel therapeutics. The current standard for assessment of wound healing in this model is histomorphometric analysis, which is labor intensive, time consuming, and requires multiple biological and technical replicates in addition to assessment of different time points. Optical Coherence Tomography (OCT) is an emerging non-invasive imaging technology originally developed for non-invasive retinal scans that avoids the deleterious effects of tissue processing. This study investigated OCT as a novel method for assessing re-epithelialization in the human ex vivo wound model. Excisional ex vivo wounds were created, maintained at air-liquid interface, and healing progression was assessed at days 4 and 7 with OCT and histology. OCT provided adequate resolution to identify the epidermis, the papillary and reticular dermis, and importantly, migrating epithelium in the wound bed. We have deployed OCT as a non-invasive tool to produce, longitudinal “optical biopsies” of ex vivo human wound healing process, and we established an optimal quantification method of re-epithelialization based on en face OCT images of the total wound area. Pairwise statistical analysis of OCT and histology based quantifications for the rate of epithelialization have shown the feasibility and superiority of OCT technology for non-invasive monitoring of human wound epithelialization. Furthermore, we have utilized OCT to evaluate therapeutic potential of allogeneic adipose stem cells revealing their ability to promote re-epithelialization in human ex vivo wounds. OCT technology is promising for its applications in wound healing and evaluation of novel therapeutics in both the laboratory and the clinical settings.
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影响因子：
10.3
作者：
Greaves, N. S.;Benatar, B.;Bayat, A.
通讯作者：
Bayat, A.
影响因子：
6.5
作者：
Roupe, K. Markus;Nybo, Mads;Sorensen, Ole E.
通讯作者：
Sorensen, Ole E.
影响因子：
3.3
作者：
Nie, Chunlei;Yang, Daping;Zhang, Jiewu
通讯作者：
Zhang, Jiewu
影响因子：
5.6
作者：
Lee, B;Vouthounis, C;Tomic-Canic, M
通讯作者：
Tomic-Canic, M
影响因子：
2.9
作者：
Mendoza-Garcia, Jenifer;Sebastian, Anil;Bayat, Ardeshir
通讯作者：
Bayat, Ardeshir