Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin- and CaM kinase-like-1-positive tuft cells.
Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin- and CaM kinase-like-1-positive tuft cells.
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DOI：
10.5009/gnl13237
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发表时间：
2014-09
期刊：
影响因子：
3.4
通讯作者：
Tateno T
中科院分区：
文献类型：
--
作者：
Mutoh H;Sashikawa M;Sakamoto H;Tateno T
双皮质素和钙调素激酶样-1（DCAMKL 1）是干细胞的标志物，主要表达在肠的隐窝基底。然而，DCAMKL 1阳性细胞已被证明是分化的簇细胞，而不是静止的祖细胞。簇状细胞是正常肠上皮中唯一表达环氧化酶2（考克斯-2）的上皮细胞。我们先前产生Cdx 2转基因小鼠作为肠上皮化生和胃癌的模型小鼠。本研究旨在探讨考克斯-2和DCAMKL 1在胃癌中的表达及其相关性。我们研究了临床样本（早期胃高分化腺癌）和Cdx 2转基因小鼠胃癌中考克斯-2和DCAMKL 1表达之间的相关性;以及使用免疫组织化学和定量实时聚合酶链反应检测DCAMKL 1转基因小鼠胃。在人和Cdx 2转基因小鼠的胃癌中，表达考克斯-2的细胞呈散在表达，而非弥漫表达。DCAMKL 1阳性细胞在胃癌中也呈散在分布，提示胃癌中仍有簇状细胞存在。考克斯-2在Cdx 2和DCAMKL 1转基因小鼠胃中的DCAMKL 1阳性簇细胞中表达，而Sox 9转录因子在胃癌中广泛表达，包括考克斯-2阳性细胞。胃癌中考克斯-2在表达DCAMKL 1的静止簇细胞中表达。
Doublecortin and CaM kinase-like-1 (DCAMKL1) is a marker of stem cells expressed predominantly in the crypt base in the intestine. However, DCAMKL1-positive cells have been shown to be differentiated tuft cells rather than quiescent progenitors. Tuft cells are the only epithelial cells that express cyclooxygenase 2 (COX-2) in the normal intestinal epithelium. We previously generated Cdx2-transgenic mice as model mice for intestinal metaplasia and gastric carcinoma. In the current study, we investigated the association between COX-2 and DCAMKL1 in gastric carcinoma. We examined the association between COX-2 and DCAMKL1 expression in gastric carcinomas in clinical samples (early gastric well-differentiated adenocarcinoma) and Cdx2-transgenic mice; and the DCAMKL1-transgenic mouse stomach using immunohistochemistry and quantitative real-time polymerase chain reaction. The COX-2-expressing cells were scattered, not diffusely expressed, in gastric carcinomas from humans and Cdx2-transgenic mice. DCAMKL1-positive cells were also scattered in the gastric carcinomas, indicating that tuft cells could still be present in gastric carcinoma. COX-2 was expressed in DCAMKL1-positive tuft cells in Cdx2- and DCAMKL1-transgenic mouse stomachs, whereas the Sox9 transcription factor was ubiquitously expressed in gastric carcinomas, including COX-2-positive cells. COX-2 is expressed in DCAMKL1-expressing quiescent tuft cells in gastric carcinoma.
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DOI：
10.1083/jcb.200311021
发表时间：
2004-07-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blache P;van de Wetering M;Duluc I;Domon C;Berta P;Freund JN;Clevers H;Jay P
通讯作者：
Jay P
影响因子：
2.5
作者：
Bezencon, C.;Fuerholz, A.;Damak, S.
通讯作者：
Damak, S.
DOI：
10.1073/pnas.0800668105
发表时间：
2008-03-18
影响因子：
11.1
作者：
Giannakis, Marios;Chen, Swaine L.;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.
影响因子：
5.2
作者：
May, Randal;Sureban, Sripathi M.;Hoang, Nguyet;Riehl, Terrence E.;Lightfoot, Stan A.;Ramanujam, Rama;Wyche, James H.;Anant, Shrikant;Houchen, Courtney W.
通讯作者：
Houchen, Courtney W.
影响因子：
5
作者：
Colnot, S;Niwa-Kawakita, M;Perret, C
通讯作者：
Perret, C