Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell
Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell
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虹鳟鱼卵巢3,β-羟基类固醇脱氢酶/Δ5-4-异构酶：其cDNA克隆和在哺乳动物细胞中表达的酶的特性
DOI：
10.1016/0014-5793(94)00795-0
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Y. Nagahama
中科院分区：
文献类型：
--
作者：
N. Sakai;Minoru Tanaka;Mika Takahashi;S. Fukada;J. Ian Mason;Y. Nagahama
从虹鱼卵巢卵泡膜细胞基因文库中克隆了编码3β-羟基类固醇脱氢酶/Δ5-4-异构酶(3β-HSD)的基因。Southern杂交结果表明，虹鱼基因组中存在编码3个β-hsd的单基因，基因组大小小于4kb。该基因与虹鳟鱼卵巢中分离的单一种mRNA杂交，1.4kb转录本在鲑鱼卵巢中含量最高，在卵子发生的后期。在非激素性猴肾肿瘤细胞中表达的鲑鱼3β-HSD具有独特的3β-HSD酶活性。脱氢表雄酮是鲑鱼3号β-HSD比17号α-羟基孕烯醇酮更受欢迎的底物。有趣的是，在COS-1细胞中表达的鲑鱼3β-HSD显示出极低的将孕烯醇酮转化为孕酮的能力。
A cDNA clone encoding 3β-hydroxysteroid dehydrogenase/Δ5-4-isomerase (3β-HSD) was isolated from a cDNA library of rainbow trout ovarian thecal cells. Southern hybridization analysis of trout genomic DNA with the cDNA suggested the presence of a single gene encoding 3β-HSD in the rainbow trout showing a total genomic size of less than 4 kilobases (kb). The cDNA hybridized to a single species of mRNA isolated from rainbow trout ovaries; the 1.4 kb transcripts were most abundant in trout ovaries during the later stages of oogenesis. The trout 3β-HSD expressed in non-steroidogenic monkey kidney tumor (COS-1) cells showed a unique enzymatic 3β-HSD activity. Dehydroepiandrosterone was a more favoral substrate of the trout 3β-HSD than 17α-hydroxypregnenolone. Interestingly, the trout 3β-HSD expressed in COS-1 cells exhibited minimal ability to convert pregnenolone to progesterone.
影响因子：
2.6
作者：
J. Ma;E. Katz;J. M. Belote
通讯作者：
J. Ma;E. Katz;J. M. Belote