Charge and sequence effects on the self-assembly and subsequent hydrogelation of Fmoc-depsipeptides.
Charge and sequence effects on the self-assembly and subsequent hydrogelation of Fmoc-depsipeptides.
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DOI：
10.1039/c4sm00009a
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发表时间：
2014-04-21
期刊：
影响因子：
3.4
通讯作者：
Suggs LJ
中科院分区：
文献类型：
--
作者：
Nguyen MM;Eckes KM;Suggs LJ
在此，我们报告了一系列 Fmoc-缩酚肽自组装成纳米纤维和水凝胶的情况。我们表明，纤维形成发生在缩酚肽结构中，其中芴基紧密相连，并且侧链电荷和序列影响自组装和随后凝胶化的程度。使用荧光发射光谱和圆二色性，我们表明可以在水凝胶形成之前在这些缓慢凝胶化系统中监测和观察自组装。我们还证明，含盐溶液的离子强度会影响自组装导致本体溶液凝胶化的时间。通过透射电子显微镜，我们报告形态变化随着时间的推移而进展，并且在凝胶化开始之前观察到胶束转变为纤维。在盐存在的情况下，凝胶化缩酚肽的降解速度比非凝胶化样品慢，而凝胶和溶液样品在水中的水解即使在 14 天后也是最小的。我们的工作表明，虽然在肽主链中掺入酯官能团减少了可用于形成和稳定超分子组装体的氢键位点的数量，但这种取代并不会阻止自组装和随后的凝胶化。
Herein we report on the self-assembly of a family of Fmoc-depsipeptides into nanofibers and hydrogels. We show that fiber formation occurs in depsipeptide structures in which the fluorenyl group is closely associated and that side-chain charge and sequence affect the extent of self-assembly and subsequent gelation. Using fluorescence emission spectroscopy and circular dichroism, we show that self-assembly can be monitored and is observed in these slow-gelling systems prior to hydrogel formation. We also demonstrate that the ionic strength of salt-containing solutions affects the time at which self-assembly results in gelation of the bulk solution. From transmission electron microscopy, we report that morphological changes progress over time and are observed as micelles transitioning to fibers prior to the onset of gelation. Gelled depsipeptides degraded at a slower rate than non-gelled samples in the presence of salt, while hydrolysis in water of both gels and solution samples was minimal even after 14 days. Our work shows that while incorporating ester functionality within a peptide backbone reduces the number of hydrogen bonding sites available for forming and stabilizing supramolecular assemblies, the substitution does not prohibit self-assembly and subsequent gelation.
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影响因子：
3.4
作者：
Saiani, A.;Mohammed, A.;Miller, A. F.
通讯作者：
Miller, A. F.
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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