Preparative chromatography of proteins analysis of the multivalent ion-exchange formalism.
Preparative chromatography of proteins analysis of the multivalent ion-exchange formalism.
复制标题
多价离子交换形式的蛋白质分析的制备色谱法。
DOI:
10.1016/s0021-9673(00)94779-4
复制
发表时间:
1988
期刊:
影响因子:
--
通讯作者:
Horváth,C
中科院分区:
文献类型:
--
作者:
Velayudhan,A;Horváth,C
Multivalent ion exchange is proposed as a generally applicable formalism to describe the non-linear chromatographic adsorption of biopolymers. Single- and multi-component isotherms are calculated that explicitly account for the influence of the mobile phase modulator concentration. Three regions of binding (strong, intermediate, and weak) are distinguished on the basis of strength of interaction, and the potential advantages of operating in the strong region for preparative purposes are pointed out. The inapplicability of the Langmuir isotherm in this region is demonstrated, and separation schemes that take advantage of the characteristic features of biopolymeric adsorption are described. The rectangular single-component ion-exchange isotherms are shown to reduce in the presence of competition to reversible concave-down forms.
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DOI:
10.1016/s0021-9673(00)94825-8
发表时间:
1986
期刊:
Journal of chromatography
影响因子:
--
作者:
A. Velayudhan;C. Horváth
通讯作者:
C. Horváth
影响因子:
1.7
作者:
A. Liao;Z. Rassi;D. LeMaster;C. Horváth
通讯作者:
C. Horváth
DOI:
10.1016/s0021-9673(00)94780-0
发表时间:
1988
期刊:
Journal of chromatography
影响因子:
--
作者:
Lee,AL;Liao,AW;Horváth,C
通讯作者:
Horváth,C
DOI:
10.1016/0021-9673(86)80059-0
发表时间:
1986
期刊:
Journal of chromatography
影响因子:
--
作者:
Lu,XM;Benedek,K;Karger,BL
通讯作者:
Karger,BL
DOI:
--
发表时间:
1955
期刊:
影响因子:
--
作者:
A. Tiselius
通讯作者:
A. Tiselius