A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells.
A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells.
复制标题
四种肌肉调节因子蛋白的独特表达模式将体细胞与胚胎、胎儿和新生小鼠肌原细胞区分开来。
作者：
T. Smith;Nancy E. Block;S. Rhodes;S. F. Konieczny;J. Miller
四种肌肉调节因子(MRF)蛋白的独特表达模式被发现在体外将早期躯体细胞与胚胎、胎儿和新生儿肢体肌源性细胞区分开来。用免疫细胞化学方法检测了肌球蛋白重链(MHC)、MyoD、Mygenin、Myf-5和MRF4蛋白在四种不同类型的小鼠成肌细胞培养中的表达：体细胞(E8.5)、胚胎(E11.5)、胎儿(E16.5)和新生肢体。在胚胎、胎儿和新生儿培养中，MRF蛋白的表达模式大致相似：MyoD是第一个表达的MRF；MyoD和mygenin在比Myf-5或MRF4更多的细胞中表达；四种MRF中的每一种都在表达MHC的细胞和不表达MHC的细胞中发现。相比之下，在体细胞培养中，Myf-5比MyoD或Mygenin首先表达，并且表达的细胞比MyoD或Mygenin多；未检测到MRF4；并且从未发现MRF与MHC在同一细胞中共同表达。因此，一些体细胞在缺乏可检测到的MRF蛋白表达的情况下，有意想不到的能力维持MHC的表达。因此，胚胎、胎儿和新生儿的肌源性细胞的不同的肌肉生成程序并不是定性上不同的MRF表达模式的简单结果，而体细胞的肌肉生成确实包括一种独特的MRF表达模式。
A unique pattern of expression of the four muscle regulatory factor (MRF) proteins was found to distinguish early somitic from embryonic, fetal and newborn limb myogenic cells in vitro. Expression of the myosin heavy chain (MHC), MyoD, myogenin, Myf-5, and MRF4 proteins was examined by immunocytochemistry in cultures of four distinct types of mouse myogenic cells: somitic (E8.5), embryonic (E11.5), fetal (E16.5) and newborn limb. In embryonic, fetal and newborn cultures, the MRF proteins were expressed in generally similar patterns: MyoD was the first MRF expressed; MyoD and myogenin were expressed by more cells than Myf-5 or MRF4; and each of the four MRFs was found both in cells that expressed MHC and in cells that did not express MHC. In cultures of somitic cells, in contrast, Myf-5 was expressed first and by more cells than MyoD or myogenin; MRF4 was not detected; and the MRFs were never found to be coexpressed with MHC in the same cell. Thus, some somitic cells had the unexpected ability to maintain MHC expression in the absence of detectable MRF protein expression. The different myogenic programs of embryonic, fetal and newborn myogenic cells are not, therefore, a simple result of qualitatively different MRF expression patterns, whereas myogenesis by somitic cells does include a unique pattern of MRF expression.
影响因子：
56.9
作者：
CHEN, L;KRAUSE, M;FIRE, A
通讯作者：
FIRE, A
影响因子：
5.3
作者：
GOSSETT, LA;KELVIN, DJ;OLSON, EN
通讯作者：
OLSON, EN