Purification of keratinocyte transglutaminase and its expression during squamous differentiation.
Purification of keratinocyte transglutaminase and its expression during squamous differentiation.
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角质形成细胞转谷氨酰胺酶的纯化及其在鳞状分化过程中的表达。
DOI：
10.5555/uri:pii:0022202x89900304
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Scott M. Thacher
中科院分区：
文献类型：
--
作者：
Scott M. Thacher
培养的人表皮角质形成细胞表达谷氨酰胺转胺酶，这种酶只在正常细胞分化的后期出现在表皮中（Thacher和Rice， cell 40:685, 1985）。使用单克隆抗体亲和柱，角化细胞转谷氨酰胺酶已从鳞状细胞癌细胞颗粒部分的非离子洗涤剂提取物中纯化了约1000倍。纯化物质中突出的92千道尔顿带在Western Blots上被单克隆抗体识别，该单克隆抗体也可以免疫沉淀活性酶。对92千道尔顿带的进一步分析表明，它显然与组织转谷氨酰胺酶没有抗原性关系，也与培养的角质形成细胞中表达的细胞质酶没有抗原性关系，后者具有免疫交叉反应，与组织转谷氨酰胺酶具有相同的分子量。在猴掌和足底表皮匀浆中，谷氨酰胺转肽类酶的活性与角化细胞酶的活性一样，表现为膜结合和阴离子交换。它可以沉淀，至少部分，与特定的单克隆抗体直接针对角化细胞转谷氨酰胺酶的帮助。经免疫组织化学测定，该酶在培养细胞中最可能的膜位置是质膜，这与角化细胞转谷氨酰胺酶在交联包膜形成中的功能一致。我们比较了大腿、新生儿包皮、银屑病表皮和猴子食道的角化细胞转谷氨酰胺酶染色模式。与正常表皮相比，培养的表皮细胞和银屑病表皮均表现出早熟表达，这表明在表皮再生成熟或过度增殖过程中，角化细胞转谷氨酰胺酶的表达可以得到实质性的改变。
Cultured human epidermal keratinocytes express a transglutaminase which appears in epidermis only during later stages of normal cell differentiation (Thacher and Rice, Cell 40:685, 1985). Using a monoclonal antibody affinity column, the keratinocyte transglutaminase has been purified approximately 1000-fold from non-ionic detergent extracts of the particulate fraction of a squamous cell carcinoma line. The prominent 92-kilodalton band in the purified material is recognized on Western Blots by a monoclonal antibody which also can immunoprecipitate the active enzyme. Further analysis of the 92-kilodalton band shows that it is apparently not antigenically related to tissue transglutaminase, or to the cytoplasmic enzyme expressed in cultured keratinocytes which is immunologically cross-reactive and of identical molecular weight to tissue transglutaminase. In monkey palmar and plantar epidermal homogenates significant transglutaminase activity appears to be membrane bound and chromatographs on anion-exchange as the keratinocyte enzyme does. It can be precipitated, at least in part, with the aid of specific monoclonal antibodies directed to keratinocyte transglutaminase. As determined immunohistochemically, the most likely membrane location of the enzyme in cultured cells is the plasma membrane, consistent with keratinocyte transglutaminase function in cross-linked envelope formation. Thigh, neonatal foreskin, psoriatic epidermis, and monkey esophagus are compared as to patterns of staining for keratinocyte transglutaminase. Cultured epidermal cells and psoriatic epidermis both show precocious expression when compared to normal epidermis, demonstrating that keratinocyte transglutaminase expression can be substantially modified during epidermal regenerative maturation or hyperproliferation.
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影响因子：
6.5
作者：
MURPHY, GF;FLYNN, TC;PINKUS, GS
通讯作者：
PINKUS, GS
高分子量人表皮转谷氨酰胺酶。
DOI：
10.1111/1523-1747.ep12275357
发表时间：
1985
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Negi,M;Colbert,MC;Goldsmith,LA
通讯作者：
Goldsmith,LA
大鼠上皮细胞角质形成细胞 I 型转谷氨酰胺酶的诱导。
DOI：
10.1111/j.1432-0436.1986.tb00418.x
发表时间：
1986
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Parenteau,NL;Pilato,A;Rice,RH
通讯作者：
Rice,RH
类维生素A抑制培养的人表皮癌细胞中的转谷氨酰胺酶活性和包膜能力。
DOI：
10.1111/j.1432-0436.1985.tb00296.x
发表时间：
1985
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Thacher,SM;Coe,EL;Rice,RH
通讯作者：
Rice,RH
大鼠毛囊和表皮转谷氨酰胺酶。
DOI：
10.1016/0005-2744(81)90150-9
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Peterson,LL;Buxman,MM
通讯作者：
Buxman,MM