Real-time assessment of cardiac perfusion, coronary angiography, and acute intravascular thrombi using dual-channel near-infrared fluorescence imaging.
Real-time assessment of cardiac perfusion, coronary angiography, and acute intravascular thrombi using dual-channel near-infrared fluorescence imaging.
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DOI：
10.1016/j.jtcvs.2008.09.082
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发表时间：
2009-07
影响因子：
6
通讯作者：
Frangioni, John V.
中科院分区：
文献类型：
--
作者：
Tanaka, Eiichi;Chen, Frederick Y.;Flaumenhaft, Robert;Graham, Gwenda J.;Laurence, Rita G.;Frangioni, John V.
We have developed an image-guided surgery system based on invisible near-infrared (NIR) fluorescent light. Presently, the only clinically-available NIR fluorophore is indocyanine green (ICG), which fluoresces at ≈ 800 nm and is used for coronary angiography. Our objective was to determine if methylene blue (MB), already FDA-approved for other indications, has useful NIR fluorescence properties for image-guided cardiac surgery. The optical properties of MB were measured after dissolution in 100% serum. Biodistribution and clearance were quantified in organs and tissues after intravenous bolus injection of 2 mg/kg MB in N = 3 rats. Coronary arteriography and cardiac perfusion were imaged in real-time after intravenous bolus injection of 1 mg/kg MB in N = 5 pigs with coronary obstructions. Coronary angiography and acute thrombi were assessed using 800 nm fluorophores, ICG and IR-786-labeled platelets, respectively. The peak absorbance and emission of MB as a NIR fluorophore occur at 667 nm and 686 nm, respectively. After intravenous injection, MB provides highly sensitive coronary angiography. A lipophilic cation, MB is extracted rapidly into tissue, with myocardium displaying unusually high uptake. MB permits real-time visualization and quantitative assessment of myocardial perfusion. Because of absent spectral overlap, use of two independent fluorophores on our imaging system permits simultaneous quantification of perfusion, venous drainage and/or intravascular thrombi. MB is an effective NIR fluorophore that provides direct visualization of coronary arteriography and cardiac perfusion. In conjunction with ≈ 800 nm NIR fluorophores, important functional assessments during cardiac surgery are also possible.
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影响因子：
158.5
作者：
CHESEBRO, JH;CLEMENTS, IP;DANIELSON, GK
通讯作者：
DANIELSON, GK
影响因子：
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Tanaka, Eiichi;Choi, Hak Soo;Frangioni, John V.
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Frangioni, John V.
影响因子：
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Tanaka, Eiichi;Choi, Hak Soo;Frangioni, John V.
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Frangioni, John V.
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作者：
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通讯作者：
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作者：
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Mohr, FW