Differential expression and regulation of prohibitin during curcumin-induced apoptosis of immortalized human epidermal HaCaT cells.
Differential expression and regulation of prohibitin during curcumin-induced apoptosis of immortalized human epidermal HaCaT cells.
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姜黄素诱导永生化人表皮HaCaT细胞凋亡过程中抑制素的差异表达及调控
DOI：
10.3892/ijmm.2014.1621
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发表时间：
2014-03
影响因子：
5.4
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Yang HB;Song W;Chen LY;Li QF;Shi SL;Kong HY;Chen P
Prohibitin(PHB)又称抑制素，在细胞的增殖、分化和凋亡过程中起重要作用。这种蛋白定位于线粒体的内膜，在那里它扮演伴侣蛋白的角色，也在细胞核中发现，在那里它负向调节转录。PHB在细胞增殖中的肿瘤抑制作用似乎是矛盾的。在本研究中，我们调查了PHB在整个细胞和核基质中的存在、定位和表达的变化，并分析了其与相关基因表达产物的共定位。Western印迹分析结果表明，pHb存在于细胞核基质蛋白的组成中，经姜黄素(1，7-bis(4-hydroxy-3-methoxyphenyl)-1，6-heptadiene-3，5-dione)处理后，pHb在整个细胞中的表达水平显著增加，而在核基质中的表达水平明显降低。激光共聚焦扫描显微镜结果显示，PHB与p53、c-Myc、Bax和Fas在HaCaT细胞中共定位，并且这一共定位区域因姜黄素处理而转移。此外，GST下拉实验结果表明，PHB与P53、c-Myc和Bax在体外有直接相互作用，但不能与Fas相互作用。本研究结果证实，核基质蛋白PHB的表达和分布影响HaCaT细胞的凋亡及其与细胞凋亡相关基因产物的共定位。
Prohibitin (PHB), also known as inhibin, is important in cell proliferation, differentiation and apoptosis. This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription. The tumor-suppressive role of PHB in cell proliferation appears to be contradictory. In this study, we investigated the existence, localization and alterations in the expression of PHB in the whole cell and nuclear matrix and analyzed its co-localization with the expression products of related genes. The western blot analysis results revealed that PHB exists in the composition of nuclear matrix proteins and that the expression level of PHB is significantly increased in the whole cell and markedly decreased in the nuclear matrix after curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) treatment. The laser confocal scanning microscope results demonstrated the co-localization of PHB with p53, c-Myc, Bax, and Fas in HaCaT cells, and this co-localization region was transferred as a result of curcumin treatment. In addition, the results of the GST pull-down assay demonstrated the direct interaction of PHB with p53, c-Myc and Bax but not Fas in vitro. Results of the present study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis.
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DOI：
10.1523/jneurosci.2849-11.2012
发表时间：
2012-01-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Zhou P;Qian L;D'Aurelio M;Cho S;Wang G;Manfredi G;Pickel V;Iadecola C
通讯作者：
Iadecola C
影响因子：
9.7
作者：
Watson, Jane L.;Hill, Richard;Hoskin, David W.
通讯作者：
Hoskin, David W.
影响因子：
4.8
作者：
Fusaro, G;Dasgupta, P;Chellappan, S
通讯作者：
Chellappan, S
影响因子：
4.2
作者：
Li, Xihai;Yei, Hongzhi;Liu, Xianxiang
通讯作者：
Liu, Xianxiang
影响因子：
2.8
作者：
Zhu, Brian;Zhai, Jianjun;Zhu, Haining;Kyprianou, Natasha
通讯作者：
Kyprianou, Natasha