A putative N-terminal nuclear export sequence is sufficient for Mps1 nuclear exclusion during interphase.
A putative N-terminal nuclear export sequence is sufficient for Mps1 nuclear exclusion during interphase.
复制标题
假定的 N 端核输出序列足以在间期期间进行 Mps1 核排除。
DOI：
10.1186/s12860-015-0048-6
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发表时间：
2015-02-27
期刊：
影响因子：
--
通讯作者：
Xu Q
中科院分区：
文献类型：
--
作者：
Jia H;Zhang X;Wang W;Bai Y;Ling Y;Cao C;Ma RZ;Zhong H;Wang X;Xu Q
Mps1是有丝分裂检查点的重要组成部分，也是一个重要的间期调节因子，在DNA损伤反应、胞质分裂和中心体复制中发挥作用。Mps1主要存在于细胞质中，并在G2期晚期重新定位到细胞核中。到目前为止，Mps1在细胞质和细胞核之间易位的机制还不清楚。在这项工作中，揭示了间期Mps1从细胞核到细胞质的动态输出过程-这一过程被CRM1抑制剂Leptomycin B阻断，表明Mps1的输出是CRM1依赖的。与这一推测一致的是，发现了Mps1和CRM1之间的直接联系。此外，通过对Mps1基序的分析，在Mps1的N端发现了一个可能的核输出序列(PNES)。该基序与经典的NES序列具有很高的相似性，与EGFP的融合导致融合蛋白从细胞核中戏剧性地被排除在外。此外，与主要存在于细胞质中的野生型蛋白相比，通过用丙氨酸取代亮氨酸，Mps1突变体失去了PNES的完整性，从而产生了弥漫性的亚细胞分布。综合这些发现，得出结论：PNES序列足以在间期从原子核输出Mps1。本文的在线版本(doi：10.1186/s12860-0150048-6)包含补充材料，授权用户可以使用。
Mps1, an essential component of the mitotic checkpoint, is also an important interphase regulator and has roles in DNA damage response, cytokinesis and centrosome duplication. Mps1 predominantly resides in the cytoplasm and relocates into the nucleus at the late G2 phase. So far, the mechanism underlying the Mps1 translocation between the cytoplasm and nucleus has been unclear. In this work, a dynamic export process of Mps1 from the nucleus to cytoplasm in interphase was revealed- a process blocked by the Crm1 inhibitor, Leptomycin B, suggesting that export of Mps1 is Crm1 dependent. Consistent with this speculation, a direct association between Mps1 and Crm1 was found. Furthermore, a putative nuclear export sequence (pNES) motif at the N-terminal of Mps1 was identified by analyzing the motif of Mps1. This motif shows a high sequence similarity to the classic NES, a fusion of this motif with EGFP results in dramatic exclusion of the fusion protein from the nucleus. Additionally, Mps1 mutant loss of pNES integrity was shown by replacing leucine with alanine which produced a diffused subcellular distribution, compared to the wild type protein which resides predominantly in cytoplasm. Taken these findings together, it was concluded that the pNES sequence is sufficient for the Mps1 export from nucleus during interphase. The online version of this article (doi:10.1186/s12860-015-0048-6) contains supplementary material, which is available to authorized users.
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影响因子：
44.1
作者：
Dou, Z;Sawagechi, A;Yao, XB
通讯作者：
Yao, XB
DOI：
10.1083/jcb.201003038
发表时间：
2010-10-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jelluma N;Dansen TB;Sliedrecht T;Kwiatkowski NP;Kops GJ
通讯作者：
Kops GJ
DOI：
10.1083/jcb.200102093
发表时间：
2001-09-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sudakin V;Chan GK;Yen TJ
通讯作者：
Yen TJ
影响因子：
3.3
作者：
Liu, ST;Chan, GKT;Yen, TJ
通讯作者：
Yen, TJ
影响因子：
4.8
作者：
Wei, JH;Chou, YF;Shieh, SY
通讯作者：
Shieh, SY