Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation
Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation
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佛波酯、TPA 刺激后，寡能间充质干细胞样克隆变得多核
DOI：
10.1002/ar.20590
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
H. Sawada
中科院分区：
文献类型：
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作者：
Keiichiro Yoshida;M. Ono;Tatsuo Maejima;M. Esaki;H. Sawada
我们建立了一个间充质干细胞克隆，5 F9 A，从大鼠骨髓基质贴壁细胞通过反复有限稀释。细胞具有成纤维细胞形状，并与相邻细胞形成紧密接触，具有类似于半融合培养物中粘附和紧密连接的交错和连接。通过RT-PCR和FACS对这些细胞的表型分析表明，它们类似于间充质干细胞，并且这些细胞可以在体外适当条件下分化为脂肪细胞和成骨细胞，显示其寡能性。此外，通过TPA（12-o-tetradecanoylphorbol 13-acetate）刺激诱导细胞成为多核细胞。Anat Rec，290：1256-1267，2007。© 2007 Wiley利斯公司
We established a mesenchymal stem cell clone, 5F9A, from rat bone marrow substrate adherent cells by repeated limiting dilutions. The cells have a fibroblastic shape and form intimate contacts with adjacent cells with interdigitations and junctions similar to adherence and tight junctions in a semi‐confluent culture. Analysis of the phenotypes of these cells by RT‐PCR and FACS demonstrated that they resembled mesenchymal stem cells, and the cells could differentiate into adiopocytes and osteoblasts under appropriate conditions in vitro showing their oligopotency. Furthermore, the cells were induced to become multinuclear cells by TPA (12‐o‐tetradecanoylphorbol 13‐acetate) stimulation. Anat Rec, 290:1256–1267, 2007. © 2007 Wiley‐Liss, Inc.
在体外培养中用佛波醇肉豆蔻酸酯乙酸酯诱导人血液来源的单核细胞形成多核巨细胞。
DOI：
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发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hassan,NF;Kamani,N;Meszaros,MM;Douglas,SD
通讯作者：
Douglas,SD
影响因子：
20.3
作者：
Reyes, M;Lund, T;Verfaillie, CM
通讯作者：
Verfaillie, CM