Screening and molecular cloning of a protective antigen from the midgut of Haemaphysalis longicornis.
Screening and molecular cloning of a protective antigen from the midgut of Haemaphysalis longicornis.
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DOI：
10.3347/kjp.2013.51.3.327
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Hu Y;Zhang J;Yang S;Wang H;Zeng H;Zhang T;Liu J
接种疫苗被认为是控制扁虱感染的一种有前途的替代方案。用SDS-PAGE分离长角血蜱中肠蛋白，并将其转移到聚偏二氟乙烯(PVDF)膜上，筛选出对咬伤有保护作用的蛋白。免疫印迹结果显示92 kDa蛋白具有免疫原性(P92)。经LC-MS/MS分析，其P92氨基酸序列为副肌球蛋白(hl-Pmy)。通过快速扩增cDNAEnds(RACE)获得hl-Pmy的全长cDNA3‘非翻译区，全长2,783bp。硬蜱副肌球蛋白(Pmy)的序列比对表明，hl-Pmy在硬蜱中具有很高的保守性。与其他无脊椎动物Pmy的保护性表位序列比较，推测hl-Pmy的保护性表位是一个与hl-Pmy蛋白N端接近的多肽(LEEAEGSSETVVEMNKKRDTE)。二级结构分析表明，Lee在α-螺旋结构中具有非螺旋链段。这些结果为开发一种针对咬人的长角绦虫的疫苗提供了基础。
Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an α-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks.
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影响因子：
2.6
作者：
Rachinsky, Anna;Guerrero, Felix D.;Scoles, Glen A.
通讯作者：
Scoles, Glen A.
影响因子：
2.4
作者：
Jongejan, F;Uilenberg, G
通讯作者：
Uilenberg, G
影响因子：
8
作者：
King, RD;Sternberg, MJE
通讯作者：
Sternberg, MJE
影响因子：
4.8
作者：
MAROTO, M;ARREDONDO, JJ;CERVERA, M
通讯作者：
CERVERA, M
中国一种新型布尼亚病毒引起的发热伴血小板减少症。
DOI：
10.1056/nejmoa1010095
发表时间：
2011-04-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Yu XJ;Liang MF;Zhang SY;Liu Y;Li JD;Sun YL;Zhang L;Zhang QF;Popov VL;Li C;Qu J;Li Q;Zhang YP;Hai R;Wu W;Wang Q;Zhan FX;Wang XJ;Kan B;Wang SW;Wan KL;Jing HQ;Lu JX;Yin WW;Zhou H;Guan XH;Liu JF;Bi ZQ;Liu GH;Ren J;Wang H;Zhao Z;Song JD;He JR;Wan T;Zhang JS;Fu XP;Sun LN;Dong XP;Feng ZJ;Yang WZ;Hong T;Zhang Y;Walker DH;Wang Y;Li DX
通讯作者：
Li DX