Comparison of Oxidation Products from DNA Components by γ-Irradiation and Fenton-Type Reactions
Comparison of Oxidation Products from DNA Components by γ-Irradiation and Fenton-Type Reactions
复制标题
γ 辐射和 Fenton 型反应 DNA 成分氧化产物的比较
DOI：
10.1269/jrr.38.121
复制
发表时间：
1997
影响因子：
2
通讯作者：
H. Kasai
中科院分区：
文献类型：
--
作者：
N. Murata‐Kamiya;H. Kamiya;M. Muraoka;H. Kaji;H. Kasai
在中性条件下，用Fenton型试剂、Fe(II)-EDTA或肾癌物质Fe(II)-NTA对四个2‘-脱氧核糖核苷进行伽马辐照或需氧处理。反应混合物立即用反相高效液相色谱法进行分析。检测到的主要产物为2-羟基脱氧腺苷(2-OH-da)、8，5‘-环脱氧腺苷(cleco-da)、8-羟基脱氧腺苷(8-OH-da)。5-甲酰基脱氧尿苷(5-CHO-DU)、5-羟基脱氧胞苷(5-OH-DC)、8-羟基脱氧鸟苷(8-OH-DG)、8，5‘-环脱氧鸟苷(clo-DG)、乙二醛及其与DG的加合物。这些氧化产物的比例因所用试剂的不同而有很大变化。例如，2-OH-da是Fe(II)-EDTA最有效的修饰核苷，而5-Cho-Du和5-OH-DC分别是Fe(II)-NTA处理和伽马辐照的主要产物。用Fe(II)-EDTA处理乙二醛的频率最高(8-OH-DG的13倍)，但用Fe(II)-NTA或伽马辐照不能检测到乙二醛的形成。8-OH-da不是由Fe-EDTA或Fe-NTA产生的，而是由伽马辐照产生的。相反，2-OH-da不是由伽马辐射产生的。这些结果表明，在不同类型的氧化应激作用下，细胞中可能产生不同比例的2-OH-da、环-da、8-OH-da、环-DG、5-CHO-DU、5-OH-DG、乙二醛-DG和8-OH-DG等三磷酸盐，并参与诱变和致癌。
The four 2'-deoxyribonucleosides were gamma-irradiated or were aerobically treated with Fenton-type-reagents, Fe(II)-EDTA or a renal carcinogen Fe(II)-nitrilotriacetic acid (NTA) under the neutral conditions. The reaction mixtures were immediately analyzed by reverse-phase HPLC. Major products detected were 2-hydroxydeoxyadenosine (2-OH-dA), 8,5'-cyclodeoxyadenosine (cyclo-dA), 8-hydroxydeoxyadenosine (8-OH-dA). 5-formyldeoxyuridine (5-CHO-dU), 5-hydroxydeoxycytidine (5-OH-dC), 8-hydroxydeoxyguanosine (8-OH-dG), 8,5'-cyclodeoxyguanosine (cyclo-dG), and glyoxal and its adduct with dG. Ratio of these oxidized products were dramatically changed depending upon the agents used. For example, 2-OH-dA was a modified nucleoside produced most efficiently by Fe(II)-EDTA, while 5-CHO-dU and 5-OH-dC were the major products by the Fe(II)-NTA treatment and gamma-irradiation, respectively. Glyoxal itself was estimated to be produced most frequently (13 folds of 8-OH-dG) when treated with Fe(II)-EDTA, but its formation was not detected by the treatment with Fe(II)-NTA or by gamma-irradiation. 8-OH-dA was not produced by Fe-EDTA or Fe-NTA but was produced by gamma-irradiation. In contrast, 2-OH-dA was not produced by gamma-irradiation. These results suggest that triphosphates of 2-OH-dA, cyclo-dA, 8-OH-dA, cyclo-dG, 5-CHO-dU, 5-OH-dC, and glyoxal-dG as well as 8-OH-dG may be produced in cells with different ratio by various types of oxidative stress and involved in mutagenesis and carcinogenesis.
登录
查看更多内容
影响因子：
56.9
作者：
AMES, BN
通讯作者：
AMES, BN
DOI：
10.1073/pnas.90.3.1122
发表时间：
1993-02-01
影响因子：
11.1
作者：
MORIYA, M
通讯作者：
MORIYA, M
DOI：
10.1073/pnas.91.14.6609
发表时间：
1994-07-05
影响因子：
11.1
作者：
FEIG, DI;SOWERS, LC;LOEB, LA
通讯作者：
LOEB, LA
影响因子：
14.9
作者：
PURMAL, AA;KOW, YW;WALLACE, SS
通讯作者：
WALLACE, SS
影响因子：
2.9
作者：
WOOD, ML;DIZDAROGLU, M;ESSIGMANN, JM
通讯作者：
ESSIGMANN, JM