In situ hybridization for epidermal growth factor receptor (EGFR) external domain transcripts in prostatic adenocarcinoma
In situ hybridization for epidermal growth factor receptor (EGFR) external domain transcripts in prostatic adenocarcinoma
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前列腺腺癌表皮生长因子受体 (EGFR) 外部域转录本的原位杂交
DOI：
10.1002/jcla.1860070310
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发表时间：
1993
影响因子：
2.7
通讯作者：
J. Tomaszewski
中科院分区：
文献类型：
--
作者：
K. Montone;J. Tomaszewski
我们采用与EGFR mRNA 5'端互补的24个碱基的合成生物素标记寡核苷酸探针，通过原位杂交检测了19例福尔马林固定的前列腺癌根治性切除术标本中的EGFR。使用25倍物镜通过光学显微镜检查所有载玻片。每个视野给出三个值：1）Gleason分级（1 - 5），2）核分级[小（<5.0 μ）、中等（5 - 10 μ）、大（>10 μ）]和3）EGFR染色强度评分（0，不存在; 1，弱; 2 +，中度至强）。共观察了851例前列腺癌的25 ×视野。所有癌症均表现出至少一定程度的细胞质EGFR信息。EGFR强度评分与肿瘤核大小相关性最好。与Gleason分级无相关性。在良性腺体的基底细胞层、高级别前列腺上皮内瘤变、间质结节、移行上皮、尿道周围腺体和神经节细胞中也发现了细胞质染色。使用未标记的EGFR探针的竞争性杂交实验显示杂交信号明显减弱，而使用生物素标记的EGFR正义探针的原位杂交为阴性。13例肿瘤中，EGFR单克隆抗体免疫组化染色仅在1/13和10/13的肿瘤中显示染色。EGFR表达在高核级别肿瘤中最为突出。进一步的研究将是必要的，以探讨这种生长因子作为这种肿瘤的预后变量。© 1993 Wiley‐利斯公司
We examined prostatic adenocarcinomas from 19 formalin fixed radical prostatectomy specimens for EGFR by in situ hybridization employing a 24 base synthetic biotin‐labeled oligonucleotide probe complementary to the 5' end of EGFR mRNA. All slides were examined by light microscopy using a 25 × objective. Each field was given three values: 1) Gleason grade (1‐5), 2) Nuclear grade [small (<5.0 μ), intermediate (5‐10 μ), large (>10 μ)], and 3) EGFR staining intensity score (0, absent; 1, weak; 2 +, moderate to strong). A total 851 25 × fields of prostatic adenocarcinoma were studied. All cancers demonstrated at least some degree of cytoplasmic EGFR message. The EGFR intensity score correlated best with tumor nuclear size. No correlation with Gleason grade was observed. Cytoplasmic staining was also identified in the basal cell layer of benign glands, high grade prostatic intraepithelial neoplasia, stromal nodules, transitional epithelium, periurethral glands, and ganglion cells. Competitive hybridization experiments using an unlabeled EGFR probe showed markedly diminished hybridization signal, while in situ hybridization with a biotin‐labeled EGFR sense probe was negative. Immunohistochemistry on 13 of the tumors with 2 monoclonal antibodies against EGFR showed staining in only 1/13 and 10/13 tumors. EGFR expression appears to be most prominent in tumors of high nuclear grade. Further studies will be necessary to explore this growth factor as a prognostic variable in this tumor. © 1993 Wiley‐Liss, Inc.
影响因子：
11.2
作者：
R. Radinsky;C. Bucana;L. Ellis;R. Sanchez;K. Cleary;D. Brigati;I. Fidler
通讯作者：
R. Radinsky;C. Bucana;L. Ellis;R. Sanchez;K. Cleary;D. Brigati;I. Fidler
影响因子：
11.2
作者：
J. B. Santon;M. Cronin;C. Macleod;J. Mendelsohn;H. Masui;G. Gill
通讯作者：
J. B. Santon;M. Cronin;C. Macleod;J. Mendelsohn;H. Masui;G. Gill
通过免疫组织化学方法对人前列腺癌、前列腺上皮内瘤变和良性增生中的表皮生长因子受体进行定位。
影响因子：
4.6
作者：
Maygarden,SJ;Strom,S;Ware,JL
通讯作者：
Ware,JL