RNA-Seq analysis of differentially expressed genes of Staphylococcus epidermidis isolated from postoperative endophthalmitis and the healthy conjunctiva
RNA-Seq analysis of differentially expressed genes of Staphylococcus epidermidis isolated from postoperative endophthalmitis and the healthy conjunctiva
复制标题
术后眼内炎和健康结膜分离表皮葡萄球菌差异表达基因的RNA-Seq分析
DOI：
10.1038/s41598-020-71050-6
复制
发表时间：
2020-08
影响因子：
4.6
通讯作者：
Huang Yusen
中科院分区：
文献类型：
--
作者：
Liu Qing;Chen Nan;Chen Huabo;Huang Yusen
Staphylococcus epidermidis (S. epidermidis) is one of the primary pathogens in postoperative endophthalmitis, which is a devastating complication of cataract surgery and often results in irreversible visual loss and even blindness. Meanwhile, it is the most frequently isolated commensal bacterium in the healthy conjunctiva. In this study, we investigated the differentially expressed genes (DEGs) of S. epidermidis isolated from the patients with postoperative endophthalmitis and the healthy conjunctiva to predict their functions and pathways by Illumina high-throughput RNA sequencing. Using genome-wide transcriptional analysis, 281 genes (142 upregulated and 139 downregulated genes) were found to be differentially expressed (fold change ≥ 2, p ≤ 0.05) in the strains from endophthalmitis. Ten randomly selected DEGs were further validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). GO enrichment analysis suggested that more DEGs were associated with the thioredoxin system and iron ion metabolism. KEGG pathway analysis revealed that more DEGs were associated with the pathways of the two-component system and pyruvate metabolism. Moreover, the gene SE1634 code for staphylococcal toxin was significantly upregulated in S. epidermidis strains of the endophthalmitis, which might be directly responsible for the pathogenesis of endophthalmitis. In conclusion, this research is helpful for further investigations on genes or pathways related with the pathogenesis and therapeutic targets of S. epidermidis endophthalmitis.
登录
查看更多内容
通过银离子靶向金黄色葡萄球菌的硫氧还蛋白还原酶 - 硫氧还蛋白系统
影响因子：
4.6
作者：
Liao, Xiangwen;Yang, Fang;Sun, Hongzhe
通讯作者：
Sun, Hongzhe
金黄色葡萄球菌对中央代谢物丙酮酸反应以调节毒力。
影响因子：
6.4
作者：
Harper L;Balasubramanian D;Ohneck EA;Sause WE;Chapman J;Mejia-Sosa B;Lhakhang T;Heguy A;Tsirigos A;Ueberheide B;Boyd JM;Lun DS;Torres VJ
通讯作者：
Torres VJ
DOI：
10.1016/j.bbagen.2016.03.013
发表时间：
2016-06-01
影响因子：
3
作者：
Gustafsson, Tomas N.;Osman, Harer;Holmgren, Arne
通讯作者：
Holmgren, Arne
影响因子：
4.2
作者：
Chiquet, Christophe;Musson, Cecile;Maurin, Max
通讯作者：
Maurin, Max
影响因子：
3.7
作者：
Flores-Páez LA;Zenteno JC;Alcántar-Curiel MD;Vargas-Mendoza CF;Rodríguez-Martínez S;Cancino-Diaz ME;Jan-Roblero J;Cancino-Diaz JC
通讯作者：
Cancino-Diaz JC