Enzyme recruitment and its role in metabolic expansion.
Enzyme recruitment and its role in metabolic expansion.
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DOI：
10.1021/bi401667f
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发表时间：
2014-02-11
期刊：
影响因子：
2.9
通讯作者：
Miller, Brian G.
中科院分区：
文献类型：
--
作者：
Schulenburg, Cindy;Miller, Brian G.
尽管距离最后一个普遍的共同祖先已经过去了109年，但蛋白质尚未达到分歧的极限。因此，代谢的复杂性不断扩大。识别和理解驱动和限制蛋白质序列空间分歧的机制不仅影响研究分子进化的进化生物学家，也影响寻求设计有用催化剂和设计新代谢途径的合成生物学家。过去50年的研究表明，为新功能募集酶是获得新代谢能力的关键事件。在这篇综述中，我们概述了遗传机制，使招聘和总结的知识现状，现有的催化剂，促进招聘的功能特性。我们还强调了最近的例子酶招聘，无论是从历史记录提供的遗传学和酶的进化实验。最后，我们展望了未来，这承诺从分子进化理论，实验室指导的进化和合成生物学的融合丰硕的成果。
Although more than 109 years have passed since the existence of the last universal common ancestor, proteins have yet to reach the limits of divergence. As a result, metabolic complexity is ever expanding. Identifying and understanding the mechanisms that drive and limit the divergence of protein sequence space impact not only evolutionary biologists investigating molecular evolution but also synthetic biologists seeking to design useful catalysts and engineer novel metabolic pathways. Investigations over the past 50 years indicate that the recruitment of enzymes for new functions is a key event in the acquisition of new metabolic capacity. In this review, we outline the genetic mechanisms that enable recruitment and summarize the present state of knowledge regarding the functional characteristics of extant catalysts that facilitate recruitment. We also highlight recent examples of enzyme recruitment, both from the historical record provided by phylogenetics and from enzyme evolution experiments. We conclude with a look to the future, which promises fruitful consequences from the convergence of molecular evolutionary theory, laboratory-directed evolution, and synthetic biology.
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影响因子：
64.8
作者：
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发表时间：
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DOI：
10.1073/pnas.0406744102
发表时间：
2005-01-18
影响因子：
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通讯作者：
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