Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
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DOI：
10.1371/journal.pone.0073995
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Allen RJ
中科院分区：
文献类型：
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作者：
Black SL;Dawson A;Ward FB;Allen RJ
尽管全球微生物生物圈的大部分被认为存在于高压环境中，但静水压力对微生物生理的影响仍然知之甚少。我们使用全基因组筛选方法，结合一种新的高通量高压细胞培养方法，研究静水压力对体内微生物生理的影响。筛选大肠杆菌K-12单基因缺失突变体Keio集合，在0.1 MPa至60 MPa的压力范围内生长。结果鉴定出在30 MPa条件下生长所需产物的6个基因为rodZ、holC、priA、dnaT、dedD和tatC，以及在40 MPa条件下生长所需产物的3个基因为tolB、rffT和iscS。我们的研究结果支持了压力对细胞生理的影响是多方面的，DNA复制、细胞分裂、细胞骨架和细胞包膜生理都是在高压生长过程中细胞生理的潜在失效点。
Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell culture method, to investigate the effects of hydrostatic pressure on microbial physiology in vivo. The Keio collection of single-gene deletion mutants in Escherichia coli K-12 was screened for growth at a range of pressures from 0.1 MPa to 60 MPa. This led to the identification of 6 genes, rodZ, holC, priA, dnaT, dedD and tatC, whose products were required for growth at 30 MPa and a further 3 genes, tolB, rffT and iscS, whose products were required for growth at 40 MPa. Our results support the view that the effects of pressure on cell physiology are pleiotropic, with DNA replication, cell division, the cytoskeleton and cell envelope physiology all being potential failure points for cell physiology during growth at elevated pressure.
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影响因子：
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作者：
BOURNS, B;FRANKLIN, S;SALMON, ED
通讯作者：
SALMON, ED
影响因子：
2.8
作者：
Dubuisson, JF;Vianney, A;Lazzaroni, JC
通讯作者：
Lazzaroni, JC
影响因子：
3.7
作者：
Crenshaw, HC;Allen, JA;Salmon, ED
通讯作者：
Salmon, ED
影响因子：
3.6
作者：
Cascales, E;Gavioli, M;Lloubès, R
通讯作者：
Lloubès, R
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
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