Molecular characterization of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene family from Citrus and the effect of fruit load on their expression.
Molecular characterization of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene family from Citrus and the effect of fruit load on their expression.
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DOI：
10.3389/fpls.2015.00389
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发表时间：
2015
影响因子：
5.6
通讯作者：
Sadka A
中科院分区：
文献类型：
--
作者：
Shalom L;Shlizerman L;Zur N;Doron-Faigenboim A;Blumwald E;Sadka A
我们最近发现了一个柑橘基因编码的SQUAMOSA启动子结合蛋白样（SPL）转录因子，含有一个序列互补的miR 156。SPL家族的基因已知在开花调节和阶段转换中起作用。在柑橘中，该基因的mRNA水平显着改变了果实负荷在芽中;下重的水果负荷（ON作物树），已知抑制明年开花，mRNA水平下调，而水果去除（去果），诱导明年开花，导致其上调。在目前的工作中，我们着手研究该基因的功能。我们发现柑橘SPL能够独立于光周期促进拟南芥开花，而miR 156抑制其开花促进活性。为了找出是否果负荷影响SPL家族的其他基因的表达，我们确定并分类柑橘基因组中的所有SPL成员，并研究其在芽和叶中的季节性表达模式，以及对去果的响应。结果表明，两个额外的SPL-like基因和miR 172，已知在拟南芥中由SPLs诱导，被果实负荷改变。本文还讨论了这些因素与柑桔开花载果效应之间的关系。
We recently identified a Citrus gene encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor that contained a sequence complementary to miR156. Genes of the SPL family are known to play a role in flowering regulation and phase transition. In Citrus, the mRNA levels of the gene were significantly altered by fruit load in buds; under heavy fruit load (ON-Crop trees), known to suppress next year flowering, the mRNA levels were down-regulated, while fruit removal (de-fruiting), inducing next-year flowering, resulted in its up-regulation. In the current work, we set on to study the function of the gene. We showed that the Citrus SPL was able promote flowering independently of photoperiod in Arabidopsis, while miR156 repressed its flowering-promoting activity. In order to find out if fruit load affected the expression of additional genes of the SPL family, we identified and classified all SPL members in the Citrus genome, and studied their seasonal expression patterns in buds and leaves, and in response to de-fruiting. Results showed that two additional SPL-like genes and miR172, known to be induced by SPLs in Arabidopsis, were altered by fruit load. The relationships between these factors in relation to the fruit-load effect on Citrus flowering are discussed.
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