Detection of Entamoeba histolytica immunoglobulins G and M to plasma membrane antigen by enzyme-linked immunosorbent assay
Detection of Entamoeba histolytica immunoglobulins G and M to plasma membrane antigen by enzyme-linked immunosorbent assay
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酶联免疫吸附法检测溶组织内阿米巴免疫球蛋白G和M对质膜抗原的影响
DOI：
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发表时间：
1990
影响因子：
9.4
通讯作者：
T. Jackson
中科院分区：
文献类型：
--
作者：
M. A. Sathar;B. Bredenkamp;V. Gathiram;A. Simjee;T. Jackson
应用酶联免疫吸附试验(EL ISA)检测了22例临床诊断为阿米巴肝脓肿(ALA)患者、10例阿米巴病以外的其他疾病住院患者、12例正常对照组和17例阿米巴病流行区患者的血清标本。以差速离心法分离的溶解组织内阿米巴HK9无菌培养物的质膜部分为抗原，检测特异性免疫球蛋白G(Ig G)和Ig M抗体。以血清稀释度1/100、492 nm光密度0.200和0.250作为诊断ALA的界值，其敏感度分别为91%(20/22)和95%(21/22)。在22例患者(10例住院患者和12例正常对照)中，特异性分别为95%(21/22)和91%(20/22)。五种致病性溶组性肠杆菌无症状携带者的血清检测均为阳性。在6个培养阴性对照中，有3个AGDT呈阳性，而所有6个培养阴性对照的免疫球蛋白夹心法均为阳性。6例非致病性酵母菌无症状携带者中，AGDT阳性2例，IgGELISA阳性3例。Ig G-EL ISA与AGDT具有良好的相关性(r=0.96)。6例培养阴性对照中仅1例阳性，6例非致病性溶组织埃希菌无症状携带者中仅1例阳性，说明该方法诊断ALA具有较高的特异性。认为质膜部分的使用提高了免疫球蛋白酶联免疫吸附试验的诊断能力。
Sixty-one serum specimens from 22 patients with clinically diagnosed amoebic liver abscess (ALA), 10 hospitalized patients with a variety of diseases other than amoebiasis, 12 normal healthy controls, and 17 subjects from an amoebiasis-endemic area were assayed by enzyme-linked immunosorbent assay (ELISA). The plasma membrane fraction of axenic cultures of Entamoeba histolytica HK9 separated from other subcellular fractions by differential centrifugation was used as the antigen to detect specific immunoglobulin G (IgG) and IgM antibodies. Using a single serum dilution of 1/100 and optical densities at 492 nm of 0.200 and 0.250 as the cutoff values for the IgM and IgG ELISAs, their respective sensitivities in 22 ALA patients were 91% (20 of 22) and 95% (21 of 22). In 22 patients (10 hospitalized and 12 normal healthy controls), the specificities of the IgM and IgG ELISAs were 95% (21 of 22) and 91% (20 of 22), respectively. All five asymptomatic carriers of pathogenic E. histolytica were seropositive by the IgG ELISA and the amoebic gel diffusion test (AGDT). The AGDT was positive for three of six culture-negative controls, while the IgG ELISA was positive for all six. For six asymptomatic carriers of nonpathogenic zymodemes, the AGDT was positive for two, and the IgG ELISA was positive for three. There was an excellent correlation (r = 0.96) between the IgG ELISA and the AGDT. Only one of six culture-negative controls, none of the asymptomatic carriers of pathogenic E. histolytica, and one of six carriers of nonpathogenic E. histolytica were seropositive by the IgM ELISA, thus highlighting the specificity of the IgM ELISA in the diagnosis of ALA. It is believed that the use of plasma membrane fractions has improved the diagnostic potential of the IgM ELISA.
使用单克隆抗体鉴定、表征和纯化致病性溶组织内阿米巴的 96,000 道尔顿表面抗原。
DOI：
10.1093/infdis/156.2.334
发表时间：
1987
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Torian,BE;Lukehart,SA;Stamm,WE
通讯作者：
Stamm,WE