Effect of bioactive dietary polyphenols on zinc transport across the intestinal Caco-2 cell monolayers.
Effect of bioactive dietary polyphenols on zinc transport across the intestinal Caco-2 cell monolayers.
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DOI：
10.1021/jf104260j
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发表时间：
2011-04-27
影响因子：
6.1
通讯作者：
Han O
中科院分区：
文献类型：
--
作者：
Kim EY;Pai TK;Han O
已知多酚类化合物具有许多有益健康的作用，包括清除活性氧和螯合金属（如铁和锌）的抗氧化活性。茶和红酒被认为是这些化合物的重要来源。然而，一些多酚类化合物在饮食中也会减少铁的吸收，可能还有其他微量金属的吸收。关于膳食多酚类化合物对铁以外微量元素状态的影响的信息很少。我们研究了表没食子儿茶素-3-没食子酸酯（EGCG）、绿茶提取物（GT）和葡萄籽提取物（GSE）对65Zn吸收的影响，并比较了它们对微孔膜插入生长的人肠道Caco-2细胞对55Fe吸收的影响。本研究中使用的EGCG、GT和GSE水平均在生理范围内，不影响Caco-2细胞单层的完整性。GSE显著（P < 0.05）降低了锌在细胞单层上的转运，且锌转运的减少与根尖锌吸收的减少有关。EGCG和GT对锌的吸收没有影响。相比之下，EGCG、GT和GSE中的多酚类化合物几乎完全阻断了上皮铁在单层细胞中的转运。GSE对锌的吸附效果与对铁的吸附效果差别很大。GSE通过降低根尖对锌的吸收来降低锌的吸收，而多酚类化合物通过提高根尖对铁的吸收来抑制铁的吸收。GSE对锌吸收的抑制作用与植酸盐相似。植酸显著（P < 0.05）通过降低锌的根尖吸收来降低锌的上皮转运。对锌吸收的抑制可能是由于GSE中原花青素的存在，它以高亲和力结合锌，阻断锌在肠细胞顶膜上的运输。进一步研究锌作为锌-多酚复合物和游离锌的吸收，将有助于进一步了解GSE和其他生物活性膳食多酚存在下的膳食锌吸收过程。目前的研究表明，如果一些人经常食用含有原花青素的食物，他们应该考虑自己的锌含量。然而，需要进一步的研究，特别是体内研究来证实这些结果。
Polyphenolic compounds are known to possess many beneficial health effects, including the antioxidative activities of scavenging reactive oxygen species and chelating metals, such as iron and zinc. Tea and red wine are thought to be important sources of these compounds. However, some polyphenolic compounds can also reduce the absorption of iron, and possibly other trace metals, when included in a diet. There is very little information on the effect of dietary polyphenolic compounds on the status of trace elements other than iron. We examined the effects of epigallocatechin-3-gallate (EGCG), green tea extract (GT) and grape seed extract (GSE) on the absorption of 65Zn and compared them with their effects on 55Fe absorption in human intestinal Caco-2 cells grown on microporous membrane inserts. The levels of EGCG, GT and GSE used in this study were within the physiological ranges and did not affect the integrity of the Caco-2 cell monolayers. GSE significantly (P < 0.05) reduced zinc transport across the cell monolayer, and the decreased zinc transport was associated with a reduction in apical zinc uptake. However, EGCG and GT did not alter zinc absorption. In contrast, the polyphenolic compounds in EGCG, GT and GSE almost completely blocked transepithelial iron transport across the cell monolayer. The effect of GSE on zinc absorption was very different from that on iron absorption. While GSE decreased zinc absorption by reducing apical zinc uptake, the polyphenolic compounds inhibited iron absorption by enhancing apical iron uptake. GSE inhibited zinc absorption similarly to that observed for phytate. Phytate significantly (P < 0.05) decreased transepithelial zinc transport by reducing apical zinc uptake. The inhibition of zinc absorption may be due to the presence of procyanidins in GSE, which bind zinc with high affinity and block the transport of zinc across the apical membrane of enterocytes. Further research on the absorption of zinc as zinc-polyphenol complexes and free zinc should provide further insight into the process of dietary zinc absorption in the presence of GSE and other bioactive dietary polyphenols. The present study suggests that some individuals should consider their zinc status if they regularly consume procyanidin-containing foods in their diet. However, further studies, especially in vivo studies, are needed to confirm these results.
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影响因子：
4.2
作者：
Kim, Eun-Young;Ham, Soo-Kyung;Han, Okhee
通讯作者：
Han, Okhee
影响因子：
4.2
作者：
Coudray, C;Tressol, JC;Mazur, A
通讯作者：
Mazur, A
影响因子：
6.1
作者：
Apak, R;Güçlu, K;Karademir, SE
通讯作者：
Karademir, SE
影响因子：
1.6
作者：
Afsana, K;Shiga, K;Hara, H
通讯作者：
Hara, H
DOI：
10.1016/s0005-2760(96)00122-1
发表时间：
1996-12-13
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM
影响因子：
--
作者：
Guo, QN;Zhao, BL;Xin, WJ
通讯作者：
Xin, WJ