Regeneration of the cementum and periodontal ligament using local BDNF delivery in class II furcation defects.
Regeneration of the cementum and periodontal ligament using local BDNF delivery in class II furcation defects.
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使用局部 BDNF 递送治疗 II 类分叉缺损的牙骨质和牙周膜再生。
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
P. Coelho
中科院分区：
文献类型：
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作者：
R. Jimbo;Jessica Singer;N. Tovar;Charles Marin;R. Neiva;E. Bonfante;M. Janal;H. Contamin;P. Coelho
牙周分叉缺陷通常通过放置物理屏障来解决，这可能会限制牙周伤口的再生潜力。本研究在非人灵长类动物模型中，从形态学上定量了脑源性神经营养因子（BDNF）在根分叉缺损中的再生作用。在8只非人类灵长类动物的第一和第二磨牙上创建II级分叉缺损（手术前有或没有诱导炎症）。采用开放皮瓣清创术治疗缺损，随后填充：A组; BDNF（500 µg mL-1）溶于高分子量透明质酸（HMW-HA），B组; BDNF（50 µg mL-1）溶于HMW-HA，C组;仅HMW-HA酸，D组;未填充缺损，或E组; BDNF（500 µg mL-1）溶于生理盐水。每2周通过计算机断层扫描观察牙周伤口愈合情况。在11周时，处死所有动物，并对上颌和下颌块活检进行非脱钙组织学检查。对新牙骨质（细胞和非细胞）和牙周韧带（PDL）形成进行线性测量。计算机断层扫描重建和软件定量显示所有组的骨填充均成功。然而，组织学评估表明，相对于所有其他组，500 µg mL-1 BDNF HMW-HA组的总牙周再生水平显著更高。各组间在牙骨质形成方面无显著差异。在手术前未诱导炎症的部位观察到显著更高的无细胞牙骨质形成。虽然所有组的骨填充和骨水泥生成相似，但500 µg mL-1 BDNF HMW-HA似乎最有效地修复PDL（相对于所有组，最小增加22%;相对于未填充缺损，超过200%）。© 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，106 B：1611-1617，2018。
Periodontal furcation defects are usually addressed by the placement of a physical barrier which may limit the regenerative potential of periodontal wounds. This study morphometrically quantified the regenerative effect of brain-derived neurotrophic factor (BDNF) in furcation defects in a non-human primate model. Grade II furcation defects (with and without induced inflammation prior to surgery) were created on the first and second molars of eight non-human primates. Defects were treated with open flap debridement and subsequently filled with either: Group A; BDNF (500 µg mL-1 ) in high-molecular weight-hyaluronic acid (HMW-HA), Group B; BDNF (50 µg mL-1 ) in HMW-HA, Group C; HMW-HA acid only, Group D; unfilled defect, or Group E; BDNF (500 µg mL-1 ) in saline. Periodontal wound healing was observed every 2 weeks by computed-tomography. At 11 weeks all animals were sacrificed and maxillary and mandibular block biopsies were referred for nondecalcified histology. Linear measurements of new cementum (cellular and acellular) and periodontal ligament (PDL) formation were performed. Computerized-tomography reconstruction and software quantification demonstrated successful bone fill for all groups. However, histometric assessment demonstrated significantly higher level of total periodontal regeneration for the 500 µg mL-1 BDNF HMW-HA relative to all other groups. No significant differences in cementogenesis were observed among groups. Significantly higher acellular cementum formation was observed for sites where inflammation was not induced prior to surgical procedures. While all groups experienced similar bone fill and cementogenesis, the 500 µg mL-1 BDNF HMW-HA appeared to most effectively repair PDL (minimum increase of ∼22% relative to all groups; over 200% relative to unfilled defects). © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1611-1617, 2018.
影响因子：
2
作者：
WEIGEL, PH;FULLER, GM;LEBOEUF, RD
通讯作者：
LEBOEUF, RD
影响因子：
4.1
作者：
Giannobile, WV
通讯作者：
Giannobile, WV