Three immunoassays based on monoclonal antibodies specific for prostate specific antigen (PSA), alpha-1-antichymotrypsin (ACT), and the PSA-ACT complex.
Three immunoassays based on monoclonal antibodies specific for prostate specific antigen (PSA), alpha-1-antichymotrypsin (ACT), and the PSA-ACT complex.
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三种免疫测定基于前列腺特异性抗原 (PSA)、α1-抗胰凝乳蛋白酶 (ACT) 和 PSA-ACT 复合物的特异性单克隆抗体。
DOI：
10.1002/pros.10247
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Kennedy,AnnR
中科院分区：
文献类型：
--
作者：
Wan,XSteven;Xu,YAnne;Ware,JeffreyH;Kennedy,AnnR
前列腺特异性抗原（PSA）已被广泛用作前列腺癌筛查和诊断的生物标志物。血清中PSA主要以与α-1-抗糜蛋白酶（ACT）的复合物形式存在，并且游离PSA和PSA-ACT复合物的测量可以提高血清PSA测定用于前列腺癌和非恶性前列腺疾病（例如良性前列腺增生（BPH））的鉴别诊断的实用性。PSA-ACT复合物通过用PSA和ACT的温育混合物免疫小鼠来产生，并通过Western印迹分析和几种酶联免疫吸附测定（ELISA）方法进行表征。游离PSA和ACT的ACT复合物。4个单克隆抗体已被选定，并利用构建三个ELISA系统，分别测量游离PSA，PSA-ACT复合物，和总PSA.CONCLUSIONSThe三个PSA检测系统在这项研究中开发的可以特异性地测量游离PSA，总PSA，和PSA-ACT复合物具有相同的摩尔灵敏度。预期这些PSA测定系统可用于前列腺癌的诊断。前列腺56：131-141，2003年。© 2003 Wiley利斯公司
BACKGROUNDProstate specific antigen (PSA) has been widely used as a biomarker for the screening and diagnosis of prostate cancer. PSA in serum predominantly exists as a complex with α‐1‐antichymotrypsin (ACT), and measurement of free PSA and the PSA–ACT complex may improve the utility of the serum PSA assay for differential diagnosis of prostate cancer and non‐malignant prostate diseases, such as benign prostatic hyperplasia (BPH).METHODSMonoclonal antibodies (MAbs) against PSA, ACT, and the PSA–ACT complex were produced by immunizing mice with an incubated mixture of PSA and ACT, and characterized by Western blot analyses and several enzyme‐linked immunosorbant assay (ELISA) methods.RESULTSThe MAbs produced in this study are capable of distinguishing the PSA–ACT complex from free PSA and ACT. Four MAbs have been selected and utilized to construct three ELISA systems for the separate measurements of free PSA, the PSA–ACT complex, and total PSA.CONCLUSIONSThe three PSA assay systems developed in this study can specifically measure free PSA, total PSA, and the PSA–ACT complex with equal molar sensitivity. It is expected that these PSA assay systems could be useful in the diagnosis of prostate cancer. Prostate 56: 131–141, 2003. © 2003 Wiley‐Liss, Inc.
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