Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C.
Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C.
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Studies of the recently discovered human rhinovirus species C (HRV-C) virus have been hampered by an inability to propagate the virus in standard cell culture. Yury Bochkov et al. have now developed a tissue culture system for HRV-C using human organ culture of sinus mucosa. This, together with development of a reverse genetics system for HRV-C, should provide a better understanding of HRV-C biology and facilitate discovery of an HRV-C receptor. The online version of this article (doi:10.1038/nm.2358) contains supplementary material, which is available to authorized users. A recently recognized human rhinovirus species C (HRV-C) is associated with up to half of HRV infections in young children. Here we propagated two HRV-C isolates ex vivo in organ culture of nasal epithelial cells, sequenced a new C15 isolate and developed the first, to our knowledge, reverse genetics system for HRV-C. Using contact points for the known HRV receptors, intercellular adhesion molecule-1 (ICAM-1) and low-density lipoprotein receptor (LDLR), inter- and intraspecies footprint analyses predicted a unique cell attachment site for HRV-Cs. Antibodies directed to binding sites for HRV-A and -B failed to inhibit HRV-C attachment, consistent with the alternative receptor footprint. HRV-A and HRV-B infected HeLa and WisL cells but HRV-C did not. However, HRV-C RNA synthesized in vitro and transfected into both cell types resulted in cytopathic effect and recovery of functional virus, indicating that the viral attachment mechanism is a primary distinguishing feature of HRV-C. The online version of this article (doi:10.1038/nm.2358) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Huang T;Wang W;Bessaud M;Ren P;Sheng J;Yan H;Zhang J;Lin X;Wang Y;Delpeyroux F;Deubel V
通讯作者：
Deubel V
DOI：
10.1183/09031936.00092410
发表时间：
2011-05
期刊：
The European respiratory journal
影响因子：
--
作者：
Bizzintino J;Lee WM;Laing IA;Vang F;Pappas T;Zhang G;Martin AC;Khoo SK;Cox DW;Geelhoed GC;McMinn PC;Goldblatt J;Gern JE;Le Souëf PN
通讯作者：
Le Souëf PN
影响因子：
5.4
作者：
Ledford, RM;Patel, NR;Pevear, DC
通讯作者：
Pevear, DC
DOI：
10.1016/j.jcv.2007.03.012
发表时间：
2007-06
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
McErlean P;Shackelton LA;Lambert SB;Nissen MD;Sloots TP;Mackay IM
通讯作者：
Mackay IM
影响因子：
11.8
作者：
Briese T;Renwick N;Venter M;Jarman RG;Ghosh D;Köndgen S;Shrestha SK;Hoegh AM;Casas I;Adjogoua EV;Akoua-Koffi C;Myint KS;Williams DT;Chidlow G;van den Berg R;Calvo C;Koch O;Palacios G;Kapoor V;Villari J;Dominguez SR;Holmes KV;Harnett G;Smith D;Mackenzie JS;Ellerbrok H;Schweiger B;Schønning K;Chadha MS;Leendertz FH;Mishra AC;Gibbons RV;Holmes EC;Lipkin WI
通讯作者：
Lipkin WI