Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome.
Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome.
复制标题
内侧皮肌节的形成需要来自背侧神经管的 Wnt 信号传导。
作者：
M. Ikeya;S. Takada
起源于体节周围组织的信号参与体节的中外侧和背腹侧图案化以及肌节的分化。Wnt-1和Wnt-3a编码富含半胱氨酸的分泌信号分子的Wnt家族成员，在发育中的神经管的背中线共表达，该区域邻近体节的背内侧部分。几条证据表明，Wnt-1和Wnt-3a具有诱导体节的内侧和背侧部分的发育以及诱导肌发生的能力。为了解决这些Wnt信号是否真的是必需的体节在正常胚胎发育过程中的发展，我们研究了体节在小鼠胚胎缺乏Wnt-1和Wnt-3a的发展。在这里，我们表明，内侧室的皮肌节没有形成和表达的外侧皮肌节标记基因，Sim-1，扩大更内侧的情况下，这些Wnt信号。此外，肌源性基因Myf-5的表达在术后9.5天降低，而许多肌源性基因的表达水平在后期似乎正常。这些结果表明，Wnt-1和Wnt-3a信号转导实际上调节真皮肌节内侧室的形成和早期肌发生。
Signals originating from tissues surrounding somites are involved in mediolateral and dorsoventral patterning of somites and in the differentiation of the myotome. Wnt-1 and Wnt-3a, which encode members of the Wnt family of cystein-rich secreted signaling molecules, are coexpressed at the dorsal midline of the developing neural tube, an area adjacent to the dorsomedial portion of the somite. Several lines of evidence indicate that Wnt-1 and Wnt-3a have the ability to induce the development of the medial and dorsal portion of somites, as well as to induce myogenesis. To address whether these Wnt signalings are really essential for the development of somites during normal embryogenesis, we investigated the development of somites in mouse embryos lacking both Wnt-1 and Wnt-3a. Here we demonstrate that the medial compartment of the dermomyotome is not formed and the expression of a lateral dermomyotome marker gene, Sim-1, is expanded more medially in the absence of these Wnt signalings. In addition, the expression of a myogenic gene, Myf-5, is decreased at 9.5 days post coitum whereas the level of expression of a number of myogenic genes in the later stage appeared normal. These results indicate that Wnt-1 and Wnt-3a signalings actually regulate the formation of the medial compartment of the dermomyotome and the early part of myogenesis.
登录
查看更多内容
Lbx1 表达和青蛙肢体发育。
影响因子：
2.4
作者：
Sabo,MichelleC;Nath,Kimberly;Elinson,RichardP
通讯作者：
Elinson,RichardP
影响因子：
10.5
作者：
Reshef, R;Maroto, M;Lassar, AB
通讯作者：
Lassar, AB
外轴肌节前体细胞的位置和生长。
DOI：
10.1242/dev.124.8.1601
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
DenetclawJr,WF;Christ,B;Ordahl,CP
通讯作者：
Ordahl,CP
背侧神经管组织皮肌切开器并在禽类胚胎中诱导轴向肌细胞。
DOI：
10.1242/dev.122.1.231
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Spence,MS;Yip,J;Erickson,CA
通讯作者：
Erickson,CA
影响因子：
10.5
作者：
McMahon, JA;Takada, S;McMahon, AP
通讯作者：
McMahon, AP