The physiological and molecular mechanisms of N transfer in Eucalyptus and Dalbergia odorifera intercropping systems using root proteomics.
The physiological and molecular mechanisms of N transfer in Eucalyptus and Dalbergia odorifera intercropping systems using root proteomics.
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DOI：
10.1186/s12870-021-02969-9
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发表时间：
2021-04-26
影响因子：
5.3
通讯作者：
Ye S
中科院分区：
文献类型：
--
作者：
Yao X;Liao L;Huang Y;Fan G;Yang M;Ye S
桉树与固氮树种（nft）混合是一种经常成功和可持续的种植做法。本研究通过评价尾巨桉（Eucalyptus urophylla × E. grandis，桉木）和一种特殊植物黄檀（Dalbergia (D.) odorifera）在间作和单作系统下的氮素转移，并对其进行蛋白质组学分析，以阐明桉树和气味黄檀混合系统中氮素转移的生理效应和分子机制。间作系统下，施氮量从臭臭木向桉树的转移率为14.61%，增加了桉树对氮的吸收和生长，抑制了臭臭木的生长。套作与单作相比，桉树和香果根分别有285个和288个差异表达蛋白，差异表达量均大于1.5倍。引进降噪瓢虫提高了桉树的抗逆性，而降噪瓢虫的抗逆性是通过提高茉莉酸（jasmonic acid， JA）水平来提高的。通过上调谷氨酰胺合成酶结节同功酶（GS）等氮代谢差异表达蛋白，增强了桉树对氮的竞争。重要的是，由于氮转运的好处，桉树中参与合成途径的蛋白质多于参与代谢途径的蛋白质，并且在桉树中发现了两组N复合转运蛋白；然而，更多的功能蛋白参与了臭草的代谢降解；其中，利用蛋白质组学分析了N向桉树转移的分子机制。我们的研究表明，氮素的转移发生在桉树上，并受到差异表达蛋白变化的影响。我们期望这些结果可以在桉树人工林可持续发展的田间试验中得到验证。在线版本包含补充材料，可在10.1186/s12870-021-02969-9获得。
The mixing of Eucalyptus with N2-fixing trees species (NFTs) is a frequently successful and sustainable cropping practice. In this study, we evaluated nitrogen (N) transfer and conducted a proteomic analysis of the seedlings of Eucalyptus urophylla × E. grandis (Eucalyptus) and an NFT, Dalbergia (D.) odorifera, from intercropping and monocropping systems to elucidate the physiological effects and molecular mechanisms of N transfer in mixed Eucalyptus and D. odorifera systems. N transfer occurred from D. odorifera to Eucalyptus at a rate of 14.61% in the intercropping system, which increased N uptake and growth in Eucalyptus but inhibited growth in D. odorifera. There were 285 and 288 differentially expressed proteins by greater than 1.5-fold in Eucalyptus and D. odorifera roots with intercropping vs monoculture, respectively. Introduction of D. odorifera increased the stress resistance ability of Eucalyptus, while D. odorifera stress resistance was increased by increasing levels of jasmonic acid (JA). Additionally, the differentially expressed proteins of N metabolism, such as glutamine synthetase nodule isozyme (GS), were upregulated to enhance N competition in Eucalyptus. Importantly, more proteins were involved in synthetic pathways than in metabolic pathways in Eucalyptus because of the benefit of N transfer, and the two groups of N compound transporters were found in Eucalyptus; however, more functional proteins were involved in metabolic degradation in D. odorifera; specifically, the molecular mechanism of the transfer of N from D. odorifera to Eucalyptus was explained by proteomics. Our study suggests that N transfer occurred from D. odorifera to Eucalyptus and was affected by the variations in the differentially expressed proteins. We anticipate that these results can be verified in field experiments for the sustainable development of Eucalyptus plantations. The online version contains supplementary material available at 10.1186/s12870-021-02969-9.
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作者：
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通讯作者：
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