Chemical carcinogens transform BHK cells by inducing a recessive mutation
Chemical carcinogens transform BHK cells by inducing a recessive mutation
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化学致癌物通过诱导隐性突变转化 BHK 细胞
DOI：
--
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发表时间：
1982
影响因子：
5.3
通讯作者：
G. di Mayorca
中科院分区：
文献类型：
--
作者：
N. Bouck;G. di Mayorca
用致突变致癌物处理BHK细胞在一个步骤中诱导肿瘤转化。这种转化显示了隐性突变所预期的特征。增加剂量的致癌物诱导的转化体的动力学类似的动力学，他们诱导6-硫代鸟嘌呤抗性或哇巴因抗性突变体在同一群体的细胞。具有温度限制表型的转化体容易被致癌物诱导，致癌物通过碱基变化引起突变，但是当使用ICR移码诱变剂时，温度限制转化体的比例与特定诱变剂诱导移码突变的频率呈负相关。在正常和转化的BHK细胞的假二倍体等基因株之间的杂交，转化表示为显性性状时，转化的父母是由乳多空病毒诱导，但被抑制为隐性性状时，转化的父母自发出现或化学诱导。在抑制的正常杂种生长时观察到转化分离，并且在大多数情况下，重新表达的转化表型是原始转化亲本的特征。
Treatment of BHK cells with mutagenic carcinogens induced neoplastic transformation in a single step. This transformation displayed the characteristics expected for a recessive mutation. Increasing doses of carcinogens induced transformants with kinetics similar to the kinetics with which they induced 6-thioguanine-resistant or ouabain-resistant mutants in the same population of cells. Transformants with temperature-restricted phenotypes were easily induced by carcinogens which cause mutations by base changes, but when ICR frameshift mutagens were used, the proportion of temperature-limited transformants was inversely related to the frequency with which a particular mutagen induced frameshift mutations. In hybrids between pseudodiploid isogenic strains of normal and transformed BHK cells, transformation was expressed as a dominant trait when the transformed parent was induced by a papovavirus, but was suppressed as a recessive trait when the transformed parent arose spontaneously or was chemically induced. Segregation of transformation was observed upon growth of suppressed normal hybrids, and the transformed phenotype which was reexpressed was in most cases characteristics of the original transformed parent.
DOI：
10.1073/pnas.77.12.7262
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KENNEDY, AR;FOX, M;LITTLE, JB
通讯作者：
LITTLE, JB
ICR-191 和甲磺酸乙酯诱导 Y5606 培养骨髓瘤细胞系中免疫球蛋白位点的突变。
DOI：
10.1016/0027-5107(81)90036-1
发表时间：
1981
期刊：
Mutation research
影响因子：
--
作者：
Wims,LA;Morrison,SL
通讯作者：
Morrison,SL