Cross-bridge and calcium behavior in ferret papillary muscle in different thyroid states.
Cross-bridge and calcium behavior in ferret papillary muscle in different thyroid states.
复制标题
不同甲状腺状态下雪貂乳头肌的跨桥和钙行为。
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
S. Kurihara
中科院分区：
文献类型：
--
作者：
N. Yagi;Y. Saeki;T. Ishikawa;S. Kurihara
用同步辐射对正常、甲亢和甲减三种不同甲状腺状态下的雪貂右心室乳头肌进行了X射线衍射研究。后两种状态分别由L-甲状腺素和他巴唑诱导。以10ms的时间分辨率记录了X射线赤道反射，以研究肌球蛋白从粗丝到细丝的质量运动。凝胶电泳法测定肌球蛋白异构体含量表明，甲亢肌中肌球蛋白以V3异构体为主，甲亢肌中肌球蛋白V1异构体占27%。用Aequorin法测定细胞内游离钙浓度。三种状态下的峰值钙浓度都是相似的，但在甲状腺功能低下状态下，达到峰值的时间较长，衰变较慢。甲状腺功能亢进症患者的收缩张力达峰时间短于正常甲状腺功能亢进症患者，而甲状腺功能减退症患者的收缩张力达峰时间长于正常甲状腺功能亢进症患者。在不同的甲状腺状态下，收缩张力的不同时间进程伴随着一种跨桥运动，这种运动与张力的发展密切相关。在甲亢患者，跨桥运动明显先于张力发育，提示甲亢肌球蛋白(V1)在向细丝附近移动和力量发育之间有较长的潜伏期。
X-ray diffraction studies were made using synchrotron radiation on ferret right ventricular papillary muscle under three different thyroid states: euthyroidism, hyperthyroidism, and hypothyroidism. The latter two states were induced by treatment with L-thyroxine and methimazole, respectively. The X-ray equatorial reflections were recorded at a time resolution of 10 ms to study the mass movement of myosin cross-bridges from thick to thin filaments. The myosin isomer content was measured by gel electrophoresis which showed that V3 isomer was predominant in euthyroid muscle and 27% of myosin was V1 isomer in hyperthyroid muscle. The intracellular free Ca concentration was measured by using the aequorin method. The peak Ca concentration was similar in all three states, but in the hypothyroid state the time-to-peak was longer and the decay was slower. The time-to-peak of twitch tension was shorter in hyperthyroidism and longer in hypothyroidism than in euthyroidism. The different time courses of twitch tension in different thyroid states accompanied a cross-bridge movement which closely followed the tension development. In hyperthyroidism, the cross-bridge movement significantly preceded tension development, suggesting that hyperthyroid myosin (V1) has a longer latency period between the shift to the vicinity of the thin filament and force development.
影响因子：
20.1
作者：
ARAI, M;OTSU, K;PERIASAMY, M
通讯作者：
PERIASAMY, M
影响因子：
20.1
作者：
KISS, E;JAKAB, G;EDES, I
通讯作者：
EDES, I
哺乳动物心室肌中的钙瞬变。
影响因子：
39.3
作者：
Allen,DG;Kurihara,S
通讯作者：
Kurihara,S