Phylogenomic analysis of UDP glycosyltransferase 1 multigene family in Linum usitatissimum identified genes with varied expression patterns.
Phylogenomic analysis of UDP glycosyltransferase 1 multigene family in Linum usitatissimum identified genes with varied expression patterns.
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DOI：
10.1186/1471-2164-13-175
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发表时间：
2012-05-08
期刊：
影响因子：
4.4
通讯作者：
Gupta VS
中科院分区：
文献类型：
--
作者：
Barvkar VT;Pardeshi VC;Kale SM;Kadoo NY;Gupta VS
糖基化过程由普遍存在的糖基转移酶(GT)家族酶催化，是一种普遍存在的对植物次生代谢物的修饰，调节激素稳态、外源物质的解毒以及次生代谢物的生物合成和储存等多种功能。亚麻(Linum usitatisimum L.)是一种商业种植的油料作物，由于其必需的脂肪酸和促进健康的木脂素而具有重要意义。亚麻UDP糖基转移酶(UGT)基因的鉴定和鉴定可以为了解这个重要基因家族提供有价值的基础信息，并有助于解释植物种子特有的糖化代谢产物积累等过程。植物基因组测序计划有助于发现该基因家族的复杂性，也为功能基因组学方法的发展铺平了道路。利用新组装的亚麻基因组草稿序列，我们利用保守的特征基序从亚麻中鉴定出137个UDP糖基转移酶(UGT)基因。对这些蛋白质序列的系统发育分析将它们聚为14个主要类群(A-N)。利用可公开获得的表达序列标签(EST)、微阵列数据和逆转录定量实时聚合酶链式反应(RT-qPCR)研究这些基因的表达模式。这些基因中的73%(137个中的100个)在15个被检查的组织中显示出表达的证据，并显示出不同的表达谱。筛选出的10个基因的RT-qPCR结果也与数字表达分析结果一致。有趣的是，发现了5个重复的UGT基因，它们在不同的组织中表现出差异表达。在检测到的7个内含子丢失/获得位置中，有2个内含子位置在大多数UGT中是保守的，尽管关于这些基因的进化还不能建立明确的关系。将亚麻UGT与其他4个测序的双子叶植物基因组中的同源基因进行比较，发现7个UGT是亚麻发散的。亚麻中含有大量的UGT基因，其中亚麻分化基因较少。系统发育分析和这些基因的表达谱确定了该作物的UGT基因的组织和条件特异性谱系。这项研究将有助于对候选基因的精确选择及其底物特异性和植物功能的进一步表征。
The glycosylation process, catalyzed by ubiquitous glycosyltransferase (GT) family enzymes, is a prevalent modification of plant secondary metabolites that regulates various functions such as hormone homeostasis, detoxification of xenobiotics and biosynthesis and storage of secondary metabolites. Flax (Linum usitatissimum L.) is a commercially grown oilseed crop, important because of its essential fatty acids and health promoting lignans. Identification and characterization of UDP glycosyltransferase (UGT) genes from flax could provide valuable basic information about this important gene family and help to explain the seed specific glycosylated metabolite accumulation and other processes in plants. Plant genome sequencing projects are useful to discover complexity within this gene family and also pave way for the development of functional genomics approaches. Taking advantage of the newly assembled draft genome sequence of flax, we identified 137 UDP glycosyltransferase (UGT) genes from flax using a conserved signature motif. Phylogenetic analysis of these protein sequences clustered them into 14 major groups (A-N). Expression patterns of these genes were investigated using publicly available expressed sequence tag (EST), microarray data and reverse transcription quantitative real time PCR (RT-qPCR). Seventy-three per cent of these genes (100 out of 137) showed expression evidence in 15 tissues examined and indicated varied expression profiles. The RT-qPCR results of 10 selected genes were also coherent with the digital expression analysis. Interestingly, five duplicated UGT genes were identified, which showed differential expression in various tissues. Of the seven intron loss/gain positions detected, two intron positions were conserved among most of the UGTs, although a clear relationship about the evolution of these genes could not be established. Comparison of the flax UGTs with orthologs from four other sequenced dicot genomes indicated that seven UGTs were flax diverged. Flax has a large number of UGT genes including few flax diverged ones. Phylogenetic analysis and expression profiles of these genes identified tissue and condition specific repertoire of UGT genes from this crop. This study would facilitate precise selection of candidate genes and their further characterization of substrate specificities and in planta functions.
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影响因子：
4.4
作者：
Fenart S;Ndong YP;Duarte J;Rivière N;Wilmer J;van Wuytswinkel O;Lucau A;Cariou E;Neutelings G;Gutierrez L;Chabbert B;Guillot X;Tavernier R;Hawkins S;Thomasset B
通讯作者：
Thomasset B
DOI：
10.1016/s1570-0232(01)00577-3
发表时间：
2002-09-25
影响因子：
3
作者：
Kraushofer, T;Sontag, G
通讯作者：
Sontag, G
影响因子：
7.4
作者：
Haberer, G;Hindemitt, T;Mayer, KFX
通讯作者：
Mayer, KFX
影响因子：
4.8
作者：
Li, Y;Baldauf, S;Bowles, DJ
通讯作者：
Bowles, DJ
影响因子：
4.2
作者：
Cullis, CA
通讯作者：
Cullis, CA