Age and diet affect gene expression profile in canine skeletal muscle.
Age and diet affect gene expression profile in canine skeletal muscle.
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DOI：
10.1371/journal.pone.0004481
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Swanson, Kelly S.
中科院分区：
文献类型：
--
作者：
Middelbos, Ingmar S.;Vester, Brittany M.;Karr-Lilienthal, Lisa K.;Schook, Lawrence B.;Swanson, Kelly S.
我们评估了基因转录在犬骨骼肌（股二头肌）使用微阵列分析，以确定年龄和饮食对基因表达的影响。使用12只雌性比格犬（6只1奥尔兹和6只12奥尔兹），给它们喂食两种实验饮食中的一种，持续12个月。一种饮食主要含有植物性蛋白质来源（PPB），而第二种饮食主要含有动物性蛋白质来源（APB）。使用Affyscore GeneChip犬基因组阵列杂交提取的RNA。年龄对基因转录的影响最大（262个差异表达基因），而饮食的影响相对较小（22个差异表达基因）。年龄（不考虑饮食）对代谢、细胞周期和细胞发育以及转录功能相关基因的影响最为显著。所有这些基因主要是下调老年犬。在PPB饮食组（165个基因中的144个）中主要注意到仅在两种饮食中的一种上差异表达的受影响基因。同样，与细胞周期（22/35）和代谢（15/19）相关的基因在老年犬中主要降低转录，但与肌肉发育相关的6/8基因表达增加。饮食对肌肉基因表达的影响主要是在老年犬，但没有观察到一致的转录模式。这些数据提供了对犬骨骼肌基因表达谱受年龄影响的见解，可以作为未来与年龄相关的肌肉疾病研究的基础。
We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet contained primarily plant-based protein sources (PPB), whereas the second diet contained primarily animal-based protein sources (APB). Affymetrix GeneChip Canine Genome Arrays were used to hybridize extracted RNA. Age had the greatest effect on gene transcription (262 differentially expressed genes), whereas the effect of diet was relatively small (22 differentially expressed genes). Effects of age (regardless of diet) were most notable on genes related to metabolism, cell cycle and cell development, and transcription function. All these genes were predominantly down-regulated in geriatric dogs. Age-affected genes that were differentially expressed on only one of two diets were primarily noted in the PPB diet group (144/165 genes). Again, genes related to cell cycle (22/35) and metabolism (15/19) had predominantly decreased transcription in geriatric dogs, but 6/8 genes related to muscle development had increased expression. Effects of diet on muscle gene expression were mostly noted in geriatric dogs, but no consistent patterns in transcription were observed. The insight these data provide into gene expression profiles of canine skeletal muscle as affected by age, could serve as a foundation for future research pertaining to age-related muscle diseases.
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影响因子：
4.6
作者：
Edwards, MG;Sarkar, D;Prolla, TA
通讯作者：
Prolla, TA
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.3
作者：
Chang, BY;Conroy, KB;Cartwright, CA
通讯作者：
Cartwright, CA
影响因子：
7.8
作者：
Beggs, ML;Nagarajan, R;Peterson, CA
通讯作者：
Peterson, CA
影响因子：
3.3
作者：
FRONTERA, WR;HUGHES, VA;EVANS, WJ
通讯作者：
EVANS, WJ