Cost-effective and compact wide-field fluorescent imaging on a cell-phone.
Cost-effective and compact wide-field fluorescent imaging on a cell-phone.
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DOI：
10.1039/c0lc00358a
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发表时间：
2011-01-21
期刊：
影响因子：
6.1
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Zhu H;Yaglidere O;Su TW;Tseng D;Ozcan A
我们演示了手机上的宽视场荧光和暗视场成像，该成像采用紧凑、重量轻、成本效益高的光学元件，机械地连接到手机现有的相机单元上。为此，我们使用电池供电的发光二极管（led）使用对接耦合从侧面泵送感兴趣的样品，其中泵送光在样品试管内被引导以均匀激发样品。然后，使用位于手机相机现有镜头正前方的另一个镜头，对样品发出的荧光进行成像。因为激发是通过垂直于我们的探测路径传播的导波发生的，一个便宜的塑料彩色滤光片足以产生荧光成像所需的暗场背景，而不需要薄膜干涉滤光片。我们通过在~81 mm2的大视场（FOV）上以2种颜色（即红色和绿色）成像各种荧光微物体来验证该平台的性能，原始空间分辨率为~20 μm。通过使用压缩采样理论，对捕获的手机图像进行额外的数字处理，我们证明了分辨率提高了2倍，在不影响视场的情况下实现了~10 μm的分辨率。此外，我们还演示了使用相同界面的非荧光标本的暗场成像，其中这次来自物体的散射光被检测而不使用任何过滤器。成像宽视场的能力对于探测大样本量（例如，100 - 0.1 mL）的血液、尿液、痰液或水是非常重要的，为此，我们还展示了全血样本中标记白细胞的荧光成像，以及水传播的致病性原生动物寄生虫，如贾第鞭毛虫囊肿。重量仅约28克（约1盎司），这种紧凑且具有成本效益的荧光成像平台连接到手机上可能非常有用，特别是在资源有限的环境中，并且可能为为全球健康应用开发的各种实验室芯片分析提供宽视场成像和定量的重要工具，例如监测HIV+患者的CD4计数或病毒载量测量。
We demonstrate wide-field fluorescent and darkfield imaging on a cell-phone with compact, light-weight and cost-effective optical components that are mechanically attached to the existing camera unit of the cell-phone. For this purpose, we used battery powered light-emitting diodes (LEDs) to pump the sample of interest from the side using butt-coupling, where the pump light was guided within the sample cuvette to uniformly excite the specimen. The fluorescent emission from the sample was then imaged using an additional lens that was positioned right in front of the existing lens of the cell-phone camera. Because the excitation occurs through guided waves that propagate perpendicular to our detection path, an inexpensive plastic colour filter was sufficient to create the dark-field background required for fluorescent imaging, without the need for a thin-film interference filter. We validate the performance of this platform by imaging various fluorescent micro-objects in 2 colours (i.e., red and green) over a large field-of-view (FOV) of ~81 mm2 with a raw spatial resolution of ~20 μm. With additional digital processing of the captured cell-phone images, through the use of compressive sampling theory, we demonstrate ~2 fold improvement in our resolving power, achieving ~10 μm resolution without a trade-off in our FOV. Further, we also demonstrate darkfield imaging of non-fluorescent specimen using the same interface, where this time the scattered light from the objects is detected without the use of any filters. The capability of imaging a wide FOV would be exceedingly important to probe large sample volumes (e.g., >0.1 mL) of e.g., blood, urine, sputum or water, and for this end we also demonstrate fluorescent imaging of labeled white-blood cells from whole blood samples, as well as water-borne pathogenic protozoan parasites such as Giardia Lamblia cysts. Weighing only ~28 g (~1 ounce), this compact and cost-effective fluorescent imaging platform attached to a cell-phone could be quite useful especially for resource-limited settings, and might provide an important tool for wide-field imaging and quantification of various lab-on-a-chip assays developed for global health applications, such as monitoring of HIV+ patients for CD4 counts or viral load measurements.
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影响因子：
7.4
作者：
Seo, Sungkyu;Isikman, Serhan O.;Sencan, Ikbal;Mudanyali, Onur;Su, Ting-Wei;Bishara, Waheb;Erlinger, Anthony;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
DOI：
10.1109/jstsp.2007.910971
发表时间：
2007-12-01
影响因子：
7.5
作者：
Kim, Seung-Jean;Koh, K.;Gorinevsky, Dimitry
通讯作者：
Gorinevsky, Dimitry
影响因子：
3.7
作者：
Granot Y;Ivorra A;Rubinsky B
通讯作者：
Rubinsky B
影响因子：
6.1
作者：
Tseng D;Mudanyali O;Oztoprak C;Isikman SO;Sencan I;Yaglidere O;Ozcan A
通讯作者：
Ozcan A
影响因子：
5.3
作者：
LUCY, LB
通讯作者：
LUCY, LB