Screening for differentially expressed genes in Anoectochilus roxburghii (Orchidaceae) during symbiosis with the mycorrhizal fungus Epulorhiza sp.
Screening for differentially expressed genes in Anoectochilus roxburghii (Orchidaceae) during symbiosis with the mycorrhizal fungus Epulorhiza sp.
复制标题
筛选金线莲（兰科）与菌根真菌 Epulorhiza sp 共生期间的差异表达基因。
DOI：
10.1007/s11427-012-4284-0
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
LI Biao,TANG Ming Juan,TANG Kun,GUO ShunXing*
中科院分区：
文献类型：
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作者：
LI Biao,TANG Ming Juan,TANG Kun,GUO ShunXing*
菌根真菌促进植物的生长发育，包括药用植物。这种促进生长的机制对农业具有理论意义和实际意义。本研究从野外生长的刺梨（Anoectochilus roxburghii）根中分离到一株内生真菌AR-18，鉴定为Epulorhiza sp.将刺梨组织培养的幼苗接种AR-18共培养60 d，形成内生菌根，明显促进刺梨的生长。为了鉴定刺梨与AR-18共生过程中差异表达的基因，我们采用差异显示反转录聚合酶链反应（DDRT-PCR）方法比较了接种真菌和对照苗的转录组。我们使用3个锚定引物和5个任意引物组成的15个引物组合扩增出52条DDRT-PCR条带，其中9条用双引物重新扩增。反向Northern blot分析进一步筛选条带。5个克隆在共生互作中表达上调，包括编码尿嘧啶磷酸糖苷转移酶的基因（UPRTs; EC 2.4.2.9）和一种假设蛋白。一个编码氨基酸跨膜转运蛋白的基因被下调，一个编码tRNA-Lys （trnK）和一个成熟酶K （matK）假基因的基因仅在接种的幼苗中表达。讨论了上述基因，特别是UPRTs和matK基因在真菌相互作用中的可能作用。本研究在刺梨中尚属首次。
Mycorrhizal fungi promote the growth and development of plants, including medicinal plants. The mechanisms by which this growth promotion occurs are of theoretical interest and practical importance to agriculture. Here, an endophytic fungus (AR-18) was isolated from roots of the orchid Anoectochilus roxburghii growing in the wild, and identified as Epulorhiza sp. Tissue-cultured seedlings of A. roxburghii were inoculated with AR-18 and co-cultured for 60 d. Endotrophic mycorrhiza formed and the growth of A. roxburghii was markedly promoted by the fungus. To identify genes in A. roxburghii that were differentially expressed during the symbiosis with AR-18, we used the differential display reverse transcription polymerase chain reaction (DDRT-PCR) method to compare the transcriptomes between seedlings inoculated with the fungus and control seedlings. We amplified 52 DDRT-PCR bands using 15 primer combinations of three anchor primers and five arbitrary primers, and nine bands were re-amplified by double primers. Reverse Northern blot analyses were used to further screen the bands. Five clones were up-regulated in the symbiotic interaction, including genes encoding a uracil phosphoribosyltransferase (UPRTs; EC 2.4.2.9) and a hypothetical protein. One gene encoding an amino acid transmembrane transporter was down-regulated, and one gene encoding a tRNA-Lys (trnK) and a maturase K (matK) pseudogene were expressed only in the inoculated seedlings. The possible roles of the above genes, especially the UPRTs and matK genes, are discussed in relation to the fungal interaction. This study is the first of its type in A. roxburghii.
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影响因子：
--
作者：
Guo Shun-xing
通讯作者：
Guo Shun-xing
DOI：
10.1385/0896034895
发表时间：
1997-07
期刊：
--
影响因子：
--
作者：
P. Liang;Jonathan D. Meade;A. Pardee
通讯作者：
P. Liang;Jonathan D. Meade;A. Pardee
影响因子：
1
作者：
R. Currah;L. Zettler;T. Mcinnis
通讯作者：
R. Currah;L. Zettler;T. Mcinnis
影响因子：
6.2
作者：
Jinfa Zhang;R. Turley;J. Stewart
通讯作者：
Jinfa Zhang;R. Turley;J. Stewart
影响因子：
56.9
作者：
LIANG, P;PARDEE, AB
通讯作者：
PARDEE, AB