Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.
Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.
复制标题
DOI：
10.1186/1471-2164-14-870
复制
发表时间：
2013-12-10
期刊：
影响因子：
4.4
通讯作者：
Rudd S
中科院分区：
文献类型：
--
作者：
Junttila S;Laiho A;Gyenesei A;Rudd S
地衣是一种与真菌和藻类或蓝藻共生的生物。地衣生活在地球上一些最恶劣的气候中，大多数地衣物种都是耐干燥的。地衣脱水耐受性已经在生物化学水平和蛋白质组学方面进行了研究，但其潜在的分子遗传机制仍有待探索。本研究的目的是研究脱水和复水对石蕊基因表达的影响。C.在脱水和再水化过程中的几个时间点收集rangiferina，并使用定制的DNA微阵列测量基因表达强度。鉴定了在一个或多个时间点差异表达的几个基因。使用qRT-PCR分析验证微阵列结果。还进行了差异表达转录物的富集分析，以确定与再水化和脱水过程最相关的基因本体论术语。我们的数据确定了数百个基因的差异表达模式，在脱水和再水化过程中调节石蕊。这些脱水和再水化事件在分子水平上彼此明显不同，并且在再水化后几分钟内观察到基因表达的最大变化。在复水的最早阶段观察到明显的变化，其机制似乎不与润湿或干燥的后期阶段共享。几个最差异表达的基因与以前研究中鉴定的基因相似，这些研究调查了其他耐干燥生物的分子机制。我们在这里提出了第一个微阵列实验的任何地衣物种，并首次研究了地衣干燥耐受性背后的遗传机制，在整个转录组水平。
Lichens are symbiotic organisms with a fungal and an algal or a cyanobacterial partner. Lichens inhabit some of the harshest climates on earth and most lichen species are desiccation-tolerant. Lichen desiccation-tolerance has been studied at the biochemical level and through proteomics, but the underlying molecular genetic mechanisms remain largely unexplored. The objective of our study was to examine the effects of dehydration and rehydration on the gene expression of Cladonia rangiferina. Samples of C. rangiferina were collected at several time points during both the dehydration and rehydration process and the gene expression intensities were measured using a custom DNA microarray. Several genes, which were differentially expressed in one or more time points, were identified. The microarray results were validated using qRT-PCR analysis. Enrichment analysis of differentially expressed transcripts was also performed to identify the Gene Ontology terms most associated with the rehydration and dehydration process. Our data identify differential expression patterns for hundreds of genes that are modulated during dehydration and rehydration in Cladonia rangiferina. These dehydration and rehydration events clearly differ from each other at the molecular level and the largest changes to gene expression are observed within minutes following rehydration. Distinct changes are observed during the earliest stage of rehydration and the mechanisms not appear to be shared with the later stages of wetting or with drying. Several of the most differentially expressed genes are similar to genes identified in previous studies that have investigated the molecular mechanisms of other desiccation-tolerant organisms. We present here the first microarray experiment for any lichen species and have for the first time studied the genetic mechanisms behind lichen desiccation-tolerance at the whole transcriptome level.
登录
查看更多内容
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.3
作者：
Dong, Chun-Hai;Hu, Xiangyang;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
6.4
作者：
Collett, H;Shen, A;Illing, N
通讯作者：
Illing, N
影响因子：
7.3
作者：
Gasulla, Franscico;Jain, Renuka;Oliver, Melvin J.
通讯作者：
Oliver, Melvin J.
影响因子：
4.4
作者：
Casteriano, Andrea;Wilkes, Meredith A.;Deaker, Rosalind
通讯作者：
Deaker, Rosalind