Biofilms as self-shaping growing nematics
Biofilms as self-shaping growing nematics
复制标题
生物膜作为自成型生长向列相
DOI：
10.1038/s41567-023-02221-1
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发表时间：
2023
期刊：
影响因子：
19.6
通讯作者：
Yan, Jing
中科院分区：
文献类型：
--
作者：
Nijjer, Japinder;Li, Changhao;Kothari, Mrityunjay;Henzel, Thomas;Zhang, Qiuting;Tai, Jung-Shen B.;Zhou, Shuang;Cohen, Tal;Zhang, Sulin;Yan, Jing
主动向列相是被动液晶的非平衡类似物。它们由消耗自由能来驱动涌现行为的各向异性单元组成。与显示器中的液晶分子一样，活性向列相的有序性和动力学对边界条件很敏感。然而，与被动液晶不同，主动向列相具有通过自生应力调节其边界的潜力。在这里，我们展示了一个三维的，活的细菌可以积极塑造自己和它的边界，通过生长诱导的应力来调节其内部结构，使用由水凝胶作为模型系统限制的细菌生物膜。我们发现，生物膜表现出急剧的过渡形状从圆顶透镜在不断变化的环境刚度或细胞-基底摩擦，这是解释了一个理论模型，认为限制和界面力之间的竞争。生长模式定义了边界的进展，这反过来又决定了细胞谱系的轨迹和空间分布。我们进一步证明，不断发展的边界和相应的应力各向异性定义的取向有序的细胞和出现的拓扑缺陷的生物膜内部。我们的研究结果可能会提供战略的发展与紧急材料性能的程序化微生物财团。
Active nematics are the non-equilibrium analogue of passive liquid crystals. They consist of anisotropic units that consume free energy to drive emergent behaviour. As with liquid crystal molecules in displays, ordering and dynamics in active nematics are sensitive to boundary conditions. However, unlike passive liquid crystals, active nematics have the potential to regulate their boundaries through self-generated stresses. Here we show how a three-dimensional, living nematic can actively shape itself and its boundary to regulate its internal architecture through growth-induced stresses, using bacterial biofilms confined by a hydrogel as a model system. We show that biofilms exhibit a sharp transition in shape from domes to lenses in response to changing environmental stiffness or cell–substrate friction, which is explained by a theoretical model that considers the competition between confinement and interfacial forces. The growth mode defines the progression of the boundary, which in turn determines the trajectories and spatial distribution of cell lineages. We further demonstrate that the evolving boundary and corresponding stress anisotropy define the orientational ordering of cells and the emergence of topological defects in the biofilm interior. Our findings may provide strategies for the development of programmed microbial consortia with emergent material properties.
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影响因子：
16.6
作者：
Zhou S;Shiyanovskii SV;Park HS;Lavrentovich OD
通讯作者：
Lavrentovich OD
对液晶拓扑缺陷核心纳米结构的实验见解。
影响因子：
8.6
作者：
Xiaoguang Wang;Young;E. Bukusoglu;Bo Zhang;Daniel S. Miller;N. Abbott
通讯作者：
N. Abbott
DOI：
10.1073/pnas.1321926111
发表时间：
2013-12
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Shuang Zhou;A. Sokolov;O. Lavrentovich;I. Aranson
通讯作者：
Shuang Zhou;A. Sokolov;O. Lavrentovich;I. Aranson
生长中的细菌菌落从单层到多层的转变。
影响因子：
8.6
作者：
Zhihong You;D. Pearce;A. Sengupta;L. Giomi
通讯作者：
L. Giomi
DOI：
10.1126/science.aaz4547
发表时间：
2020-03-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Duclos G;Adkins R;Banerjee D;Peterson MSE;Varghese M;Kolvin I;Baskaran A;Pelcovits RA;Powers TR;Baskaran A;Toschi F;Hagan MF;Streichan SJ;Vitelli V;Beller DA;Dogic Z
通讯作者：
Dogic Z