A novel parainfluenza virus type 3 (PIV3) identified from goat herds with respiratory diseases in eastern China.
A novel parainfluenza virus type 3 (PIV3) identified from goat herds with respiratory diseases in eastern China.
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DOI：
10.1016/j.vetmic.2014.08.027
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发表时间：
2014-11-07
影响因子：
3.3
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Li W;Mao L;Cheng S;Wang Q;Huang J;Deng J;Wang Z;Zhang W;Yang L;Hao F;Ding Y;Sun Y;Wei J;Jiang P;Jiang J
在许多患有呼吸道疾病的山羊农场中检测到了独特的 PIV3 菌株。通过 RT-PCR 和 HA 测定分离并确认病毒。遗传和系统发育分析表明该分离株可能是PIV3的新成员。山羊源PIV3对山羊具有致病性。 3 型副流感病毒 (PIV3) 是人类和许多动物最重要的病毒性呼吸道病原体之一，但山羊感染的报道却很少。 2013年8月开始，中国东部江苏省和安徽省的山羊出现了严重的呼吸道疾病。为了确定病原体，使用 RT-PCR 或 PCR 测试了许多相关病原体。在大多数临床鼻拭子或血清样本中检测到了独特的PIV3毒株。该病毒在 MDBK 细胞上分离，并通过 RT-PCR、核苷酸序列分析和血凝试验进行表征。使用简并引物对扩增整个 M 和 F 基因编码区、HN、5'-UTR-N 和 L 基因片段。基于这些基因的核苷酸、氨基酸序列比对和系统发育分析表明，山羊来源的PIV3毒株与之前报道的BPIV3基因型和HPIV3毒株不同。这种新型分离株名为 JS2013，可能是呼吸道病毒属的潜在新成员。山羊用 JS2013 培养物进行实验感染。接种病毒的山羊出现咳嗽和流鼻涕，这与呼吸道疾病有关。接种后 4-10 天 (dpi) 检测到病毒血症和病毒脱落。病毒特异性 HI 抗体从 14 dpi 起呈阳性。这是首次从中国山羊群中检测到PIV3，并对新型山羊源PIV3进行遗传和致病特征分析。
Unique PIV3 strain was detected from many goat farms with respiratory disease. Virus was isolated and confirmed by RT-PCR and HA assay. Genetic and phylogenetic analysis showed the isolate might be a new member of PIV3. Goat derived PIV3 had pathogenicity to goats. Parainfluenza virus type 3 (PIV3) is one of the most important viral respiratory pathogens for humans and for many animals, but goat infection has been rarely reported. Starting in Aug 2013, goats in the Jiangsu and Anhui provinces of eastern China suffered severe respiratory diseases. In order to identify the causative agent, numerous related pathogens were tested with RT-PCR or PCR. A unique PIV3 strain was detected in most of the clinical nasal swabs or serum samples. The virus was isolated on MDBK cells and characterized by RT-PCR, nucleotide sequence analysis and hemagglutination test. The entire M and F gene coding regions, HN, 5′-UTR-N and L gene fragments were amplified using pairs of degenerate primers. Nucleotide, amino acid sequence alignments and phylogenetic analyses based on these genes indicated that the goat-derived PIV3 strain was distinct from previously reported BPIV3 genotypes and HPIV3 strains. The novel isolate, named JS2013, might be a potentially new member of the respirovirus genus. Goats were experimentally infected with JS2013 culture. The virus-inoculated goats displayed coughing and nasal discharges that were related to respiratory diseases. Viremia and virus shedding were detected during 4–10 days post-inoculation (dpi). Virus-specific HI antibodies became positive from 14 dpi. This is the first report of the detection of PIV3 from Chinese goat herds and genetic and pathogenetic characterization of the novel goat-derived PIV3.
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