Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait.
Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait.
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DOI：
10.3390/v14102219
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发表时间：
2022-10-09
期刊：
影响因子：
--
通讯作者：
McKenna R
中科院分区：
文献类型：
--
作者：
Lakshmanan R;Mietzsch M;Jimenez Ybargollin A;Chipman P;Fu X;Qiu J;Söderlund-Venermo M;McKenna R
细小病毒是小的单链DNA病毒，没有包膜衣壳。确定衣壳结构为注释对病毒生命周期重要的区域提供了一个框架。阿留申水貂病病毒（AMDV）和人类细小病毒4 （PARV4）分别属于Amdoparvovirus属和Tetraparvovirus属的细小病毒，是水貂中的一种病原体。虽然由AMDV引起的阿留申水貂病是水貂养殖的主要威胁，但在人类感染PARV4后尚未确定明确的临床表现。在这里，AMDV和PARV4的衣壳结构通过冷冻电镜分别在2.37和3.12 Å分辨率下进行了测定。尽管氨基酸序列同一性较低（10-30%），但两种病毒都具有细小病毒衣壳的二十面体性质，60种病毒蛋白（VPs）通过两倍、三倍和五倍对称的vp相关相互作用组装衣壳，但当衣壳结构叠加到其他细小病毒上时，表面环显示出主要的结构变异性。AMDV和PARV4的衣壳结构将增加对细小病毒结构平台的现有认识，并允许未来对这些病毒进行功能注释，这将有助于在分子水平上了解其感染机制，从而开发诊断和治疗方法。
Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera Amdoparvovirus and Tetraparvovirus, respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10–30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics.
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影响因子：
4.7
作者：
Leisi, Remo;Di Tommaso, Chiarina;Ros, Carlos
通讯作者：
Ros, Carlos
DOI：
10.3390/v14091922
发表时间：
2022-08-30
期刊：
Viruses
影响因子：
--
作者：
Lakshmanan RV;Hull JA;Berry L;Burg M;Bothner B;McKenna R;Agbandje-McKenna M
通讯作者：
Agbandje-McKenna M
DOI：
10.3390/v13091833
发表时间：
2021-09-14
期刊：
Viruses
影响因子：
--
作者：
Markarian NM;Abrahamyan L
通讯作者：
Abrahamyan L
DOI：
10.3390/v13020330
发表时间：
2021-02-20
期刊：
Viruses
影响因子：
--
作者：
Yu JC;Mietzsch M;Singh A;Jimenez Ybargollin A;Kailasan S;Chipman P;Bhattacharya N;Fakhiri J;Grimm D;Kapoor A;Kučinskaitė-Kodzė I;Žvirblienė A;Söderlund-Venermo M;McKenna R;Agbandje-McKenna M
通讯作者：
Agbandje-McKenna M
影响因子：
5.4
作者：
Kaufmann, Baerbel;Chipman, Paul R.;Rossmann, Michael G.
通讯作者：
Rossmann, Michael G.