Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK(3/6)/p(38)MAPK and MEK(1/2)/ERK(1/2) Signalling Pathways.
Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK(3/6)/p(38)MAPK and MEK(1/2)/ERK(1/2) Signalling Pathways.
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瘦素通过 PI3K/Akt/mTOR、MKK3/6/p38MAPK 和 MEK1/2/ERK1/2 信号通路的组合刺激 Goldïsh 垂体中催乳素 mRNA 的表达
DOI：
10.3390/ijms18122781
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发表时间：
2017-12-20
影响因子：
5.6
通讯作者：
Chen T
中科院分区：
文献类型：
--
作者：
Yan A;Chen Y;Chen S;Li S;Zhang Y;Jia J;Yu H;Liu L;Liu F;Hu C;Tang D;Chen T
瘦素在脑垂体水平的作用已在哺乳动物物种中得到了广泛的研究，但在低等脊椎动物，特别是硬骨鱼中仍未得到充分的表征。催乳素(PRL)是一种对鱼类渗透压调节具有重要作用的脑下垂体激素。以金鱼为模型，研究了瘦素受体(Lepr)在全球和脑-垂体中的分布，并探讨了Lepr在不同脑垂体区的表达与主要垂体激素的关系。进一步分析重组金鱼Leptin-AI和Leptin-AII对垂体PRL mRNA表达的影响，并从药理学角度探讨瘦素诱导PRL表达的信号转导机制。我们的结果表明，金鱼LepR在脑-脑垂体区大量表达，在脑垂体区有高度重叠的PRL转录。重组金鱼Leptin-AI和Leptin-AII蛋白能以剂量和时间依赖的方式刺激金鱼脑垂体PRL mRNA的表达，无论是通过腹腔注射还是原代细胞培养。此外，PI3K/Akt/mTOR、MKK3/6/p38MAPK和MEK1/2/ERK1/2-而不是JAK2/STAT 1、3和5级联通路-参与了瘦素诱导的金鱼脑垂体PRL mRNA的表达。
Leptin actions at the pituitary level have been extensively investigated in mammalian species, but remain insufficiently characterized in lower vertebrates, especially in teleost fish. Prolactin (PRL) is a pituitary hormone of central importance to osmoregulation in fish. Using goldfish as a model, we examined the global and brain-pituitary distribution of a leptin receptor (lepR) and examined the relationship between expression of lepR and major pituitary hormones in different pituitary regions. The effects of recombinant goldfish leptin-AI and leptin-AII on PRL mRNA expression in the pituitary were further analysed, and the mechanisms underlying signal transduction for leptin-induced PRL expression were determined by pharmacological approaches. Our results showed that goldfish lepR is abundantly expressed in the brain-pituitary regions, with highly overlapping PRL transcripts within the pituitary. Recombinant goldfish leptin-AI and leptin-AII proteins could stimulate PRL mRNA expression in dose- and time-dependent manners in the goldfish pituitary, by both intraperitoneal injection and primary cell incubation approaches. Moreover, the PI3K/Akt/mTOR, MKK3/6/p38MAPK, and MEK1/2/ERK1/2—but not JAK2/STAT 1, 3 and 5 cascades—were involved in leptin-induced PRL mRNA expression in the goldfish pituitary.
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影响因子：
4
作者：
Gorissen, Marnix;Bernier, Nicholas J.;Huising, Mark O.
通讯作者：
Huising, Mark O.
影响因子：
56.9
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4.1
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