Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability.
Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability.
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DOI：
10.1111/1462-2920.13930
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发表时间：
2017-10
影响因子：
5.1
通讯作者：
Kelly DJ
中科院分区：
文献类型：
--
作者：
Guccione EJ;Kendall JJ;Hitchcock A;Garg N;White MA;Mulholland F;Poole RK;Kelly DJ
空肠弯曲菌是全球食源性细菌性胃肠炎最常见的原因，是一种微需氧菌，必须在高环境氧张力下生存，适应肠道中的氧限制并抵抗宿主氧化攻击。在这里，C.使用碳（丝氨酸）限制的连续恒化培养物以恒定生长速率研究空肠生理学。我们发现，感知有氧规模可以校准的醋酸排泄通量，成为零时，代谢是完全有氧（100%有氧）。从150%到40%有氧生活的下调实验中的转录组变化揭示了许多新的氧调节基因，并强调了电子传递链的重新建模。无标记蛋白质组学分析显示，在40%有氧条件下，许多参与宿主定殖的蛋白质（例如，PorA、CadF、FlpA、CjkT）变得更加丰富。PorA丰度在低于100%有氧条件下急剧增加。相比之下，几种柠檬酸循环酶，肽转运蛋白CstA，PEB 1天冬氨酸/谷氨酸转运蛋白，LutABC乳酸脱氢酶和PutA脯氨酸脱氢酶随着有氧活动的增加而变得更加丰富。我们还观察到氧化应激保护酶和Fe-S簇生物合成蛋白在100%有氧条件下的协调反应。我们的方法揭示了关键的毒力因子，响应有限的氧气供应和特定的转运蛋白和分解代谢途径激活增加有氧。
Campylobacter jejuni, the most frequent cause of food‐borne bacterial gastroenteritis worldwide, is a microaerophile that has to survive high environmental oxygen tensions, adapt to oxygen limitation in the intestine and resist host oxidative attack. Here, oxygen‐dependent changes in C. jejuni physiology were studied at constant growth rate using carbon (serine)‐limited continuous chemostat cultures. We show that a perceived aerobiosis scale can be calibrated by the acetate excretion flux, which becomes zero when metabolism is fully aerobic (100% aerobiosis). Transcriptome changes in a downshift experiment from 150% to 40% aerobiosis revealed many novel oxygen‐regulated genes and highlighted re‐modelling of the electron transport chains. A label‐free proteomic analysis showed that at 40% aerobiosis, many proteins involved in host colonisation (e.g., PorA, CadF, FlpA, CjkT) became more abundant. PorA abundance increased steeply below 100% aerobiosis. In contrast, several citric‐acid cycle enzymes, the peptide transporter CstA, PEB1 aspartate/glutamate transporter, LutABC lactate dehydrogenase and PutA proline dehydrogenase became more abundant with increasing aerobiosis. We also observed a co‐ordinated response of oxidative stress protection enzymes and Fe‐S cluster biogenesis proteins above 100% aerobiosis. Our approaches reveal key virulence factors that respond to restricted oxygen availability and specific transporters and catabolic pathways activated with increasing aerobiosis.
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影响因子：
5.2
作者：
Gundogdu O;da Silva DT;Mohammad B;Elmi A;Mills DC;Wren BW;Dorrell N
通讯作者：
Dorrell N
DOI：
10.1073/pnas.1701587114
发表时间：
2017-08-15
影响因子：
11.1
作者：
Khademian, Maryam;Imlay, James A.
通讯作者：
Imlay, James A.
影响因子：
4.4
作者：
Butcher, James;Handley, Rebecca A.;Stintzi, Alain
通讯作者：
Stintzi, Alain
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
3.6
作者：
Hendrixson, DR;DiRita, VJ
通讯作者：
DiRita, VJ