NMR Metabolomics Assessment of Osteogenic Differentiation of Adipose-Tissue-Derived Mesenchymal Stem Cells.
NMR Metabolomics Assessment of Osteogenic Differentiation of Adipose-Tissue-Derived Mesenchymal Stem Cells.
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DOI：
10.1021/acs.jproteome.1c00832
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发表时间：
2022-03-04
影响因子：
4.4
通讯作者：
Gil, Ana M.
中科院分区：
文献类型：
--
作者：
Bispo, Daniela S. C.;Jesus, Catarina S. H.;Correia, Marlene;Ferreira, Filipa;Bonifazio, Giulia;Goodfellow, Brian J.;Oliveira, Mariana B.;Mano, Joao F.;Gil, Ana M.
关键词：
本文介绍了，第一次，我们所知，非靶向的核磁共振（NMR）代谢组学表征的极性细胞内代谢适应的人脂肪间充质干细胞在成骨分化。使用间充质干细胞（MSC）进行骨再生是传统骨移植的一种有前途的替代方法，非靶向代谢组学可以揭示有关MSC成骨分化的新代谢信息，使其行为能够被理解和监测/指导有效的治疗。我们的研究结果揭示了30多种代谢物水平的统计学相关变化，说明了一个高度动态的过程，在整个21天的成骨分化过程中存在显著变化，主要涉及氨基酸代谢和蛋白质合成;能量代谢和糖酵解，三羧酸循环和氧化磷酸化的作用;细胞膜代谢;核苷酸代谢（包括O-糖基化中间体和NAD+的特定参与）;以及保护性抗氧化机制中的代谢参与者（如谷胱甘肽和特定氨基酸）。提出了不同的代谢阶段，并得到了所观察到的代谢物变化的假定生化解释的支持。这项工作为利用非靶向NMR代谢组学寻找成骨分化功效的潜在代谢标志物奠定了基础。
This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics may unveil novel metabolic information on the osteogenic differentiation of MSCs, allowing their behavior to be understood and monitored/guided toward effective therapies. Our results unveiled statistically relevant changes in the levels of just over 30 identified metabolites, illustrating a highly dynamic process with significant variations throughout the whole 21-day period of osteogenic differentiation, mainly involving amino acid metabolism and protein synthesis; energy metabolism and the roles of glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation; cell membrane metabolism; nucleotide metabolism (including the specific involvement of O-glycosylation intermediates and NAD+); and metabolic players in protective antioxidative mechanisms (such as glutathione and specific amino acids). Different metabolic stages are proposed and are supported by putative biochemical explanations for the metabolite changes observed. This work lays the groundwork for the use of untargeted NMR metabolomics to find potential metabolic markers of osteogenic differentiation efficacy.
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影响因子：
7.5
作者：
Gibon E;Lu L;Goodman SB
通讯作者：
Goodman SB
DOI：
10.1359/jbmr.091023
发表时间：
2010-04
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Ciancaglini P;Yadav MC;Simão AM;Narisawa S;Pizauro JM;Farquharson C;Hoylaerts MF;Millán JL
通讯作者：
Millán JL
影响因子：
4.8
作者：
Addison, William N.;Azari, Fereshteh;McKee, Marc D.
通讯作者：
McKee, Marc D.
影响因子：
4
作者：
Jun, Ji Hae;Lee, Sun-Hwan;Baek, Jeong-Hwa
通讯作者：
Baek, Jeong-Hwa
影响因子：
--
作者：
Bow, Austin;Jackson, Bailey;Dhar, Madhu
通讯作者：
Dhar, Madhu